| IED ID | IndEnz0018000372 |
| Enzyme Type ID | peroxidase000372 |
| Protein Name |
Dual oxidase EC 1.11.1.- EC 1.6.3.1 |
| Gene Name | Duox CG3131 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MSVPSAPHQRAESKNRVPRPGQKNRKLPKLRLHWPGATYGGALLLLLISYGLELGSVHCYEKMYSQTEKQRYDGWYNNLAHPDWGSVDSHLVRKAPPSYSDGVYAMAGANRPSTRRLSRLFMRGKDGLGSKFNRTALLAFFGQLVANEIVMASESGCPIEMHRIEIEKCDEMYDRECRGDKYIPFHRAAYDRDTGQSPNAPREQINQMTAWIDGSFIYSTSEAWLNAMRSFHNGTLLTEKDGKLPVRNTMRVPLFNNPVPSVMKMLSPERLFLLGDPRTNQNPAILSFAILFLRWHNTLAQRIKRVHPDWSDEDIYQRARHTVIASLQNVIVYEYLPAFLGTSLPPYEGYKQDIHPGIGHIFQAAAFRFGHTMIPPGIYRRDGQCNFKETPMGYPAVRLCSTWWDSSGFFADTSVEEVLMGLASQISEREDPVLCSDVRDKLFGPMEFTRRDLGALNIMRGRDNGLPDYNTARESYGLKRHKTWTDINPPLFETQPELLDMLKEAYDNKLDDVDVYVGGMLESYGQPGEFFTAVIKEQFQRLRDADRFWFENERNGIFTPEEIAELRKITLWDIIVNSTDVKEEEIQKDVFMWRTGDPCPQPMQLNATELEPCTYLEGYDYFSGSELMFIYVCVFLGFVPILCAGAGYCVVKLQNSKRRRLKIRQEALRAPQHKGSVDKMLAREWLHANHKRLVTVKFGPEAAIYTVDRKGEKLRTFSLKHIDVVSVEESATNHIKKKPYILLRVPSDHDLVLELESYGARRKFVKKLEDFLLLHKKEMTLMEVNRDIMLARAETRERRQKRLEYFFREAYALTFGLRPGERRRRSDASSDGEVMTVMRTSLSKAEFAAALGMKPNDMFVRKMFNIVDKDQDGRISFQEFLETVVLFSRGKTDDKLRIIFDMCDNDRNGVIDKGELSEMMRSLVEIARTTSLGDDQVTELIDGMFQDVGLEHKNHLTYQDFKLMMKEYKGDFVAIGLDCKGAKQNFLDTSTNVARMTSFNIEPMQDKPRHWLLAKWDAYITFLEENRQNIFYLFLFYVVTIVLFVERFIHYSFMAEHTDLRHIMGVGIAITRGSAASLSFCYSLLLLTMSRNLITKLKEFPIQQYIPLDSHIQFHKIAACTALFFSVLHTVGHIVNFYHVSTQSHENLRCLTREVHFASDYKPDITFWLFQTVTGTTGVMLFIIMCIIFVFAHPTIRKKAYNFFWNMHTLYIGLYLLSLIHGLARLTGPPRFWMFFLGPGIVYTLDKIVSLRTKYMALDVIDTDLLPSDVIKIKFYRPPNLKYLSGQWVRLSCTAFRPHEMHSFTLTSAPHENFLSCHIKAQGPWTWKLRNYFDPCNYNPEDQPKIRIEGPFGGGNQDWYKFEVAVMVGGGIGVTPYASILNDLVFGTSTNRYSGVACKKVYFLWICPSHKHFEWFIDVLRDVEKKDVTNVLEIHIFITQFFHKFDLRTTMLYICENHFQRLSKTSIFTGLKAVNHFGRPDMSSFLKFVQKKHSYVSKIGVFSCGPRPLTKSVMSACDEVNKTRKLPYFIHHFENFG |
| Enzyme Length | 1537 |
| Uniprot Accession Number | Q9VQH2 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Peroxidase activity is inhibited by aminotriazole and azide. {ECO:0000269|PubMed:16272120}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H(+) + NADH + O2 = H2O2 + NAD(+); Xref=Rhea:RHEA:11264, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.6.3.1; Evidence={ECO:0000269|PubMed:16272120}; CATALYTIC ACTIVITY: Reaction=H(+) + NADPH + O2 = H2O2 + NADP(+); Xref=Rhea:RHEA:11260, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.6.3.1; Evidence={ECO:0000269|PubMed:16272120}; |
| DNA Binding | |
| EC Number | 1.11.1.-; 1.6.3.1 |
| Enzyme Function | FUNCTION: Plays a role in innate immunity limiting microbial proliferation in the gut (PubMed:16272120, PubMed:25639794). Acts downstream of a hh-signaling pathway to induce the production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794). May generate antimicrobial oxidative burst through its peroxidase-like domain (PubMed:16272120). {ECO:0000269|PubMed:16272120, ECO:0000269|PubMed:25639794}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (5); Erroneous termination (1); Frameshift (1); Glycosylation (4); Metal binding (9); Modified residue (2); Region (2); Topological domain (8); Transmembrane (7) |
| Keywords | Calcium;FAD;Flavoprotein;Glycoprotein;Hydrogen peroxide;Membrane;Metal-binding;NADP;Oxidoreductase;Peroxidase;Phosphoprotein;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 826; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 1105; /note=Phosphotyrosine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 1039909; 14778558; 15020472; 15204079; 16858400; 17216353; 17246014; 17247021; 18039034; 18419806; 19289084; 19317464; 19668222; 19797770; 19958789; 20188558; 20220848; 20371351; 21074052; 21399827; 21808060; 21979942; 22242003; 22368770; 22520468; 22724070; 22817988; 22858408; 22952763; 23071443; 23394834; 23663779; 23685204; 23781070; 23808845; 23944235; 24127584; 24141879; 24380076; 24381562; 24409421; 24439372; 24455491; 24673174; 24735869; 24799191; 24819984; 24856462; 24983497; 24997434; 25254641; 25421701; 25687947; 25688716; 25945494; 26068126; 26136621; 26353752; 26431326; 26443856; 26587980; 26634780; 26655037; 26690827; 26726767; 26876781; 26878754; 26898463; 26935105; 27160597; 27262554; 27274436; 27562340; 27575697; 27923120; 28069988; 28248301; 28289197; 28462587; 28753614; 28760809; 28853394; 29049360; 29091025; 29420191; 29503179; 29540218; 29644870; 29707694; 29750169; 29752064; 29787709; 29795158; 29849035; 29952680; 30166445; 30180210; 30325949; 30353950; 30356215; 30385770; 30446385; 30540251; 30587065; 30733377; 30770378; 30822306; 30926832; 30986429; 31009489; 31068592; 31315941; 31316388; 31554796; 31575764; 31610128; 31698828; 32075729; 32078802; 32228858; 32277031; 32339931; 32561812; 32612612; 32686670; 32791328; 32917927; 33050786; 33176146; 33187802; 33400912; 33600942; 33863891; 33972730; 34126772; 34290589; 34445633; 34600048; 34622778; 4255937; 4624174; 6804094; 7707961; 8001779; 8100740; 826447; 8458732; 8782435; 9409834; |
| Motif | |
| Gene Encoded By | |
| Mass | 177,690 |
| Kinetics | |
| Metal Binding | METAL 868; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 870; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 872; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 874; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 879; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 904; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 906; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 908; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 915; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| Rhea ID | RHEA:11264; RHEA:11260 |
| Cross Reference Brenda |