Detail Information for IndEnz0018000462
IED ID IndEnz0018000462
Enzyme Type ID peroxidase000462
Protein Name Glutathione S-transferase S1
EC 2.5.1.18
GST class-sigma 1
Glutathione S-transferase 2
Gene Name GstS1 GST2 GSTS1-1 CG8938
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MADEAQAPPAEGAPPAEGEAPPPAEGAEGAVEGGEAAPPAEPAEPIKHSYTLFYFNVKALAEPLRYLFAYGNQEYEDVRVTRDEWPALKPTMPMGQMPVLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLKIAVVSYEPEDEIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIEKRPVTEV
Enzyme Length 249
Uniprot Accession Number P41043
Absorption
Active Site
Activity Regulation
Binding Site BINDING 54; /note=Glutathione; /evidence=ECO:0000269|PubMed:12547198; BINDING 85; /note=Glutathione; /evidence=ECO:0000269|PubMed:12547198; BINDING 89; /note=Glutathione; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:12547198, ECO:0000269|PubMed:22082028};
DNA Binding
EC Number 2.5.1.18
Enzyme Function FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in the detoxification of metabolites produced during cellular division and morphogenesis (PubMed:1445191, PubMed:12547198). {ECO:0000269|PubMed:12547198, ECO:0000269|PubMed:1445191, ECO:0000269|PubMed:22082028}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (5); Binding site (3); Chain (1); Domain (2); Helix (12); Region (3); Sequence conflict (3); Turn (2)
Keywords 3D-structure;Detoxification;Reference proteome;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1M0U;
Mapped Pubmed ID 10471706; 11007492; 11081516; 11320318; 11358508; 11435138; 11583575; 11746221; 11901120; 12175807; 12408803; 12443531; 12470891; 12610523; 12826097; 12932322; 14605208; 14972680; 15238523; 15238527; 15238529; 15689351; 15695583; 15710747; 15911761; 15976174; 16752200; 16980395; 17194782; 17907271; 17984172; 18039872; 18059160; 18070343; 18184789; 18428031; 18651915; 18757933; 19234471; 19342413; 19343724; 19519625; 19684592; 19863269; 20015541; 20036725; 20041201; 20371351; 20617381; 20658179; 20813047; 20816975; 21151574; 21447707; 21512585; 21799256; 22384357; 22940110; 22952763; 23000359; 23071443; 23790629; 24231732; 24625679; 24684830; 24789699; 25193493; 25310450; 25312911; 25373207; 25426014; 25794683; 25888134; 26494844; 26801178; 27274984; 27317776; 27357258; 27582081; 27794539; 28184905; 28568827; 29670218; 30001323; 30110626; 30426565; 30753230; 31018943; 31040111; 31101394; 31683705; 31722958; 32054635; 32284136; 32344153; 32345615; 33159074; 33357404; 33859668; 7683659; 8384578; 8743688; 8743700; 8798144; 9514730; 9536439; 9927183; 9927464;
Motif
Gene Encoded By
Mass 27,614
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.52 mM for glutathione {ECO:0000269|PubMed:22082028}; KM=1.24 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:22082028}; Vmax=4.53 umol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:22082028}; Vmax=2.81 umol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate {ECO:0000269|PubMed:22082028}; Vmax=84.3 nmol/min/mg enzyme with adrenochrome as substrate {ECO:0000269|PubMed:22082028}; Vmax=0.112 umol/min/mg enzyme with phenethyl isothiocyanate as substrate {ECO:0000269|PubMed:22082028};
Metal Binding
Rhea ID RHEA:16437
Cross Reference Brenda