Detail Information for IndEnz0018000478
IED ID IndEnz0018000478
Enzyme Type ID peroxidase000478
Protein Name Catalase-peroxidase
CP
EC 1.11.1.21
Catalase-2
Peroxidase/catalase
Gene Name katG cat-2 NCU05770
Organism Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Sordariales Sordariaceae Neurospora Neurospora crassa Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Enzyme Sequence MSECPVRKSNVGGGGTRNHDWWPAQLRLNILRQHTPVSNPLDKDFDYAAAFKSLDYEGLKKDLTKLMTDSQDWWPADFGHYGGLFIRMAWHSAGTYRVTDGRGGGGEGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGNKISWSDLLLLTGNVALESMGFKTFGFAGGRPDTWEADESVYWGAETTWLGNEDRYSEGQEGHEGHGVVQGDESKKQHTDIHNRDLQSPLASSHMGLIYVNPEGPDGIPDPVASAKDIRVTFGRMAMNDEETVALIAGGHSFGKTHGAGPTHHVGKEPEAAPIEHQGLGWANSFGQGKGPDTITSGLEVTWTPTPTKWGMGYLEYLYKFDWEPTKSPAGANQWVAKNAEPTIPDAYDPNKKKLPTMLTTDIALRMDPAYDKICRDYLANPDKFADAFARAWFKLLHRDMGPRTRWIGPEVPSEILPWEDYIPPVDYQIIDDNDIAALKKEILATGVAPKKLIFVAWSSASSFRGSDKRGGANGARIRLAPQNEWKVNDPSTLREVLAALESVQQKFNDSSSGKKVSLADLIVLGGVAALEQASGLVVPFTPGRNDATQEHTDVHSFTHLEPHADGFRSYGKGTKRVRTEQFLIDRASLLTLSAPELTALIGGLRVLEANYDGSSYGVLTKTPGKLTNDYFVNLLDTNTAWKAADNEGEVFIGYDRKTHDKKWTATRADLIFGAHAELRALAEVYAAVDGEEKFKRDFVAAWHKVMNLDRFDLKQEGRGQNAPKL
Enzyme Length 753
Uniprot Accession Number Q8X182
Absorption
Active Site ACT_SITE 91; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_03108
Activity Regulation ACTIVITY REGULATION: Inhibited by KCN. {ECO:0000269|PubMed:12033445}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108};
DNA Binding
EC Number 1.11.1.21
Enzyme Function FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.75 for the peroxidase reaction and 6.25 for the catalase reaction. {ECO:0000269|PubMed:12033445};
Pathway
nucleotide Binding
Features Active site (1); Beta strand (12); Chain (1); Cross-link (2); Helix (43); Metal binding (1); Region (1); Site (1); Turn (7)
Keywords 3D-structure;Cytoplasm;Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome
Interact With
Induction INDUCTION: Induced in late stationary growth phase. {ECO:0000269|PubMed:12033445}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03108}.
Modified Residue
Post Translational Modification PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_03108}.
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 5WHQ; 5WHS;
Mapped Pubmed ID 29305053;
Motif
Gene Encoded By
Mass 83,380
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13.2 mM for H(2)O(2) for the catalase reaction {ECO:0000269|PubMed:12033445};
Metal Binding METAL 279; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_03108
Rhea ID RHEA:30275; RHEA:20309
Cross Reference Brenda 1.11.1.6;