| IED ID | IndEnz0018000494 |
| Enzyme Type ID | peroxidase000494 |
| Protein Name |
L-ascorbate peroxidase 1, cytosolic APXa EC 1.11.1.11 OsAPx01 |
| Gene Name | APX1 OsI_010770 |
| Organism | Oryza sativa subsp. indica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. indica (Rice) |
| Enzyme Sequence | MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTMKTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGREDKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFCPLVEKYAADEKAFFEDYKEAHLKLSELGFADA |
| Enzyme Length | 250 |
| Uniprot Accession Number | A2XFC7 |
| Absorption | |
| Active Site | ACT_SITE 42; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Initiator methionine (1); Metal binding (5); Region (1); Sequence conflict (1); Site (1) |
| Keywords | Calcium;Cytoplasm;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Potassium;Reference proteome;Stress response |
| Interact With | |
| Induction | INDUCTION: By salt stress and hydrogen peroxide. {ECO:0000269|PubMed:15832681}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,103 |
| Kinetics | |
| Metal Binding | METAL 163; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 164; /note=Potassium; /evidence=ECO:0000250; METAL 180; /note=Potassium; /evidence=ECO:0000250; METAL 182; /note=Potassium; /evidence=ECO:0000250; METAL 187; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda |