Detail Information for IndEnz0018000610
IED ID IndEnz0018000610
Enzyme Type ID peroxidase000610
Protein Name Bacterial microcompartment shell protein PduB
Bacterial microcompartment protein homotrimer
BMC-T
Propanediol utilization protein PduB
Gene Name pduB STM2039
Organism Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Enzyme Sequence MSSNELVEQIMAQVIARVATPEQQAIPGQPQPIRETAMAEKSCSLTEFVGTAIGDTLGLVIANVDTALLDAMKLEKRYRSIGILGARTGAGPHIMAADEAVKATNTEVVSIELPRDTKGGAGHGSLIILGGNDVSDVKRGIEVALKELDRTFGDVYGNEAGHIELQYTARASYALEKAFGAPIGRACGIIVGAPASVGVLMADTALKSANVEVVAYSSPAHGTSFSNEAILVISGDSGAVRQAVTSAREIGKTVLATLGSEPKNDRPSYI
Enzyme Length 270
Uniprot Accession Number P37449
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: The two proteins produced are among the major shell proteins of the bacterial microcompartment (BMC) dedicated to 1,2-propanediol (1,2-PD) degradation (PubMed:12923081, PubMed:21239588). Required for structural integrity of BMCs and to mitigate propionaldehyde toxicity (PubMed:21239588). The N-terminal 13 residues are important for correct assembly of the BMC shell (PubMed:28138097). The isolated BMC shell component protein ratio for J:A:B':B:K:T:U is approximately 15:10:7:6:1:1:2 (PubMed:12923081). The N-terminus of the long form (PduB) is required for correct formation of BMCs, deletions in the first 37 residues have substantially reduced levels of the major lumen enzymes. May play a major role in binding the enzyme contents to the shell (PubMed:28138097). {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:21239588, ECO:0000269|PubMed:28138097}.; FUNCTION: The 1,2-PD-specific bacterial microcompartment (BMC) concentrates low levels of 1,2-PD catabolic enzymes, concentrates volatile reaction intermediates thus enhancing pathway flux and keeps the level of toxic, mutagenic propionaldehyde low. {ECO:0000305|PubMed:28475631}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Polyol metabolism; 1,2-propanediol degradation. {ECO:0000305|PubMed:10498708}.
nucleotide Binding
Features Alternative sequence (1); Chain (1); Domain (2); Erroneous initiation (1); Frameshift (1); Initiator methionine (2); Mutagenesis (9); Region (1); Sequence conflict (1)
Keywords Alternative promoter usage;Bacterial microcompartment;Direct protein sequencing;Reference proteome
Interact With
Induction INDUCTION: BMC production is induced by growth on 1,2-PD vitamin B12 medium. {ECO:0000269|PubMed:10498708}.
Subcellular Location SUBCELLULAR LOCATION: Bacterial microcompartment {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:27063436}.
Modified Residue
Post Translational Modification PTM: In purified BMCs seen as a 28.0 kDa and 25.0 kDa form, both of which have been N-terminally sequenced and whose N-fMet is removed; the smaller form is called PduB'. {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:27063436}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 28,016
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda