| IED ID | IndEnz0018000610 |
| Enzyme Type ID | peroxidase000610 |
| Protein Name |
Bacterial microcompartment shell protein PduB Bacterial microcompartment protein homotrimer BMC-T Propanediol utilization protein PduB |
| Gene Name | pduB STM2039 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MSSNELVEQIMAQVIARVATPEQQAIPGQPQPIRETAMAEKSCSLTEFVGTAIGDTLGLVIANVDTALLDAMKLEKRYRSIGILGARTGAGPHIMAADEAVKATNTEVVSIELPRDTKGGAGHGSLIILGGNDVSDVKRGIEVALKELDRTFGDVYGNEAGHIELQYTARASYALEKAFGAPIGRACGIIVGAPASVGVLMADTALKSANVEVVAYSSPAHGTSFSNEAILVISGDSGAVRQAVTSAREIGKTVLATLGSEPKNDRPSYI |
| Enzyme Length | 270 |
| Uniprot Accession Number | P37449 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The two proteins produced are among the major shell proteins of the bacterial microcompartment (BMC) dedicated to 1,2-propanediol (1,2-PD) degradation (PubMed:12923081, PubMed:21239588). Required for structural integrity of BMCs and to mitigate propionaldehyde toxicity (PubMed:21239588). The N-terminal 13 residues are important for correct assembly of the BMC shell (PubMed:28138097). The isolated BMC shell component protein ratio for J:A:B':B:K:T:U is approximately 15:10:7:6:1:1:2 (PubMed:12923081). The N-terminus of the long form (PduB) is required for correct formation of BMCs, deletions in the first 37 residues have substantially reduced levels of the major lumen enzymes. May play a major role in binding the enzyme contents to the shell (PubMed:28138097). {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:21239588, ECO:0000269|PubMed:28138097}.; FUNCTION: The 1,2-PD-specific bacterial microcompartment (BMC) concentrates low levels of 1,2-PD catabolic enzymes, concentrates volatile reaction intermediates thus enhancing pathway flux and keeps the level of toxic, mutagenic propionaldehyde low. {ECO:0000305|PubMed:28475631}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Polyol metabolism; 1,2-propanediol degradation. {ECO:0000305|PubMed:10498708}. |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Domain (2); Erroneous initiation (1); Frameshift (1); Initiator methionine (2); Mutagenesis (9); Region (1); Sequence conflict (1) |
| Keywords | Alternative promoter usage;Bacterial microcompartment;Direct protein sequencing;Reference proteome |
| Interact With | |
| Induction | INDUCTION: BMC production is induced by growth on 1,2-PD vitamin B12 medium. {ECO:0000269|PubMed:10498708}. |
| Subcellular Location | SUBCELLULAR LOCATION: Bacterial microcompartment {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:27063436}. |
| Modified Residue | |
| Post Translational Modification | PTM: In purified BMCs seen as a 28.0 kDa and 25.0 kDa form, both of which have been N-terminally sequenced and whose N-fMet is removed; the smaller form is called PduB'. {ECO:0000269|PubMed:12923081, ECO:0000269|PubMed:27063436}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,016 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |