Detail Information for IndEnz0018000662
IED ID IndEnz0018000662
Enzyme Type ID peroxidase000662
Protein Name Peroxidase 66
EC 1.11.1.7
Plasma membrane-bound peroxidase 3-2
pmPOX3-2
Gene Name PER66 POX3-2 PRX66
Organism Zea mays (Maize)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae PACMAD clade Panicoideae Andropogonodae Andropogoneae Tripsacinae Zea Zea mays (Maize)
Enzyme Sequence MAASVSASCLNRLSSLAVVLVALASAASAQLSSTFYDRSCPNALSTIRSGVNSAVRQEPRVGASLLRLHFHDCFVRGCDASLLLNDTSGEQSQGPNLTLNPRGFVVVNSIKAQVESVCPGIVSCADILAVAARDGVVALGGPSWTVLLGRRDSTASFAGQTSDLPPPTSSLGQLLSAYNKKNLNPTDMVALSGAHTIGQAQCSSFNDHIYNDTNINSAFAASLRANCPRAGSTALAPLDTTTPNAFDNAYYTNLLSQKGLLHSDQELFNSGSTDSTVRSFASSTSAFNSAFATAMVKMGNLSPQTGTQGQIRRSCWKVNS
Enzyme Length 320
Uniprot Accession Number A5H454
Absorption
Active Site ACT_SITE 71; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012"
Activity Regulation
Binding Site BINDING 165; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
DNA Binding
EC Number 1.11.1.7
Enzyme Function FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Binding site (1); Chain (1); Disulfide bond (4); Glycosylation (3); Metal binding (10); Modified residue (1); Signal peptide (1); Site (1)
Keywords Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue MOD_RES 30; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297
Post Translational Modification
Signal Peptide SIGNAL 1..29; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 20032108;
Motif
Gene Encoded By
Mass 33,419
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=54.7 umol/min/mg enzyme with guaiacol as substrate {ECO:0000269|PubMed:18603027}; Note=In the presence of H(2)O(2).;
Metal Binding METAL 72; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 75; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 77; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 79; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 81; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 195; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 196; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 239; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 242; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 247; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297
Rhea ID RHEA:56136
Cross Reference Brenda