Detail Information for IndEnz0018000695
IED ID IndEnz0018000695
Enzyme Type ID peroxidase000695
Protein Name Chorion peroxidase
EC 1.11.1.7
Peroxinectin-related protein
Dpxt
Gene Name Pxt CG7660
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MSRILFILLLLIVTQLSELQAAAFSVRQNRFDEVPDLQTPAPLATSTESSKKPEKATSGLLKKCLPCSDGIRCVPQIQCPAHVRMESHEKPQICDLPAGKFGYCCETGQNHTAPKPETSPKERRSGFPTILSPAVLDEARRNFEHLMHGVAQIPVRRGFPDFAHGLVFHSTAKDDLHNFAISNSAIEQVMTTQLFGKKEQVPVEDFITNNVPIKFTETPLAHHCQPPPVCGNIRSVYRSMDGTCNNPEPQRSLWGAAGQPMERMLPPAYEDGIWTPRAHSSDGTPLLGARKISRTLLSDVDRPHPKYNLMVMQFGQVLAHDISQTSSIRLEDGSLVQCCSPEGKVALSPQQSHFACMPIHVEPDDEFFSAFGVRCLNFVRLSLVPSPDCQLSYGKQLTKVTHFVDASPVYGSSDEASRSLRAFRGGRLRMMNDFGRDLLPLTNDKKACPSEEAGKSCFHSGDGRTNQIISLITLQILLAREHNRVAGALHELNPSASDETLFQEARRIVIAEMQHITYNEFLPIIIGPQQMKRFRLVPLHQGYSHDYNVNVNPAITNEFSGAAYRMGHSSVDGKFQIRQEHGRIDEVVNIPDVMFNPSRMRKREFYDDMLRTLYSQPMQQVDSSISQGLSRFLFRGDNPFGLDLAAINIQRGRDQGLRSYNDYLELMGAPKLHSFEQFPIEIAQKLSRVYRTPDDIDLWVGGLLEKAVEGGVVGVTFAEIIADQFARFKQGDRYYYEYDNGINPGAFNPLQLQEIRKVTLARLLCDNSDRLTLQAVPLAAFVRADHPGNQMIGCDDPNLPSVNLEAWRA
Enzyme Length 809
Uniprot Accession Number Q9VEG6
Absorption
Active Site ACT_SITE 320; /note="Proton acceptor"; /evidence="ECO:0000250|UniProtKB:P05164, ECO:0000255|PROSITE-ProRule:PRU00298"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
DNA Binding
EC Number 1.11.1.7
Enzyme Function FUNCTION: Required for ovarian follicle maturation. Involved in the formation of a rigid and insoluble egg chorion by catalyzing chorion protein cross-linking through dityrosine formation and phenol oxidase-catalyzed chorion melanization. {ECO:0000269|PubMed:18216169}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Disulfide bond (3); Erroneous termination (1); Frameshift (1); Glycosylation (1); Metal binding (1); Modified residue (1); Propeptide (1); Region (1); Sequence conflict (15); Signal peptide (1); Site (1)
Keywords Acetylation;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18216169}. Note=In the chorion layer of the mature eggs.
Modified Residue MOD_RES 224; /note=N-acetylcysteine; in Chorion peroxidase light chain; /evidence=ECO:0000250
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11404335; 11861563; 14605208; 15710747; 18433294; 19378459; 19952109; 20220848; 21074052; 21177962; 21205669; 21637834; 21979943; 22952763; 23051736; 23071443; 23316440; 23440886; 23583758; 24070373; 24284900; 24302569; 25000897; 25215488; 25341099; 25358341; 25540427; 25808493; 26353752; 26413411; 26413413; 26526100; 26551273; 26587980; 26801178; 27172210; 30753230; 31506320; 31992650; 32302036; 32432969; 33104720; 33163493;
Motif
Gene Encoded By
Mass 90,539
Kinetics
Metal Binding METAL 568; /note="Iron (heme b axial ligand)"; /evidence="ECO:0000250|UniProtKB:P05164, ECO:0000255|PROSITE-ProRule:PRU00298"
Rhea ID RHEA:56136
Cross Reference Brenda