| IED ID | IndEnz0018000695 |
| Enzyme Type ID | peroxidase000695 |
| Protein Name |
Chorion peroxidase EC 1.11.1.7 Peroxinectin-related protein Dpxt |
| Gene Name | Pxt CG7660 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MSRILFILLLLIVTQLSELQAAAFSVRQNRFDEVPDLQTPAPLATSTESSKKPEKATSGLLKKCLPCSDGIRCVPQIQCPAHVRMESHEKPQICDLPAGKFGYCCETGQNHTAPKPETSPKERRSGFPTILSPAVLDEARRNFEHLMHGVAQIPVRRGFPDFAHGLVFHSTAKDDLHNFAISNSAIEQVMTTQLFGKKEQVPVEDFITNNVPIKFTETPLAHHCQPPPVCGNIRSVYRSMDGTCNNPEPQRSLWGAAGQPMERMLPPAYEDGIWTPRAHSSDGTPLLGARKISRTLLSDVDRPHPKYNLMVMQFGQVLAHDISQTSSIRLEDGSLVQCCSPEGKVALSPQQSHFACMPIHVEPDDEFFSAFGVRCLNFVRLSLVPSPDCQLSYGKQLTKVTHFVDASPVYGSSDEASRSLRAFRGGRLRMMNDFGRDLLPLTNDKKACPSEEAGKSCFHSGDGRTNQIISLITLQILLAREHNRVAGALHELNPSASDETLFQEARRIVIAEMQHITYNEFLPIIIGPQQMKRFRLVPLHQGYSHDYNVNVNPAITNEFSGAAYRMGHSSVDGKFQIRQEHGRIDEVVNIPDVMFNPSRMRKREFYDDMLRTLYSQPMQQVDSSISQGLSRFLFRGDNPFGLDLAAINIQRGRDQGLRSYNDYLELMGAPKLHSFEQFPIEIAQKLSRVYRTPDDIDLWVGGLLEKAVEGGVVGVTFAEIIADQFARFKQGDRYYYEYDNGINPGAFNPLQLQEIRKVTLARLLCDNSDRLTLQAVPLAAFVRADHPGNQMIGCDDPNLPSVNLEAWRA |
| Enzyme Length | 809 |
| Uniprot Accession Number | Q9VEG6 |
| Absorption | |
| Active Site | ACT_SITE 320; /note="Proton acceptor"; /evidence="ECO:0000250|UniProtKB:P05164, ECO:0000255|PROSITE-ProRule:PRU00298" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; |
| DNA Binding | |
| EC Number | 1.11.1.7 |
| Enzyme Function | FUNCTION: Required for ovarian follicle maturation. Involved in the formation of a rigid and insoluble egg chorion by catalyzing chorion protein cross-linking through dityrosine formation and phenol oxidase-catalyzed chorion melanization. {ECO:0000269|PubMed:18216169}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Disulfide bond (3); Erroneous termination (1); Frameshift (1); Glycosylation (1); Metal binding (1); Modified residue (1); Propeptide (1); Region (1); Sequence conflict (15); Signal peptide (1); Site (1) |
| Keywords | Acetylation;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18216169}. Note=In the chorion layer of the mature eggs. |
| Modified Residue | MOD_RES 224; /note=N-acetylcysteine; in Chorion peroxidase light chain; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11404335; 11861563; 14605208; 15710747; 18433294; 19378459; 19952109; 20220848; 21074052; 21177962; 21205669; 21637834; 21979943; 22952763; 23051736; 23071443; 23316440; 23440886; 23583758; 24070373; 24284900; 24302569; 25000897; 25215488; 25341099; 25358341; 25540427; 25808493; 26353752; 26413411; 26413413; 26526100; 26551273; 26587980; 26801178; 27172210; 30753230; 31506320; 31992650; 32302036; 32432969; 33104720; 33163493; |
| Motif | |
| Gene Encoded By | |
| Mass | 90,539 |
| Kinetics | |
| Metal Binding | METAL 568; /note="Iron (heme b axial ligand)"; /evidence="ECO:0000250|UniProtKB:P05164, ECO:0000255|PROSITE-ProRule:PRU00298" |
| Rhea ID | RHEA:56136 |
| Cross Reference Brenda |