| IED ID |
IndEnz0018000702 |
| Enzyme Type ID |
peroxidase000702 |
| Protein Name |
Myeloperoxidase
MPO
EC 1.11.2.2
Cleaved into: Myeloperoxidase; 89 kDa myeloperoxidase; 84 kDa myeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain
|
| Gene Name |
MPO |
| Organism |
Homo sapiens (Human) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Primates
Haplorrhini
Simiiformes
Catarrhini
Hominoidea (apes)
Hominidae (great apes)
Homininae
Homo
Homo sapiens (Human)
|
| Enzyme Sequence |
MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQPSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYFKQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAYQDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVKRNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFVTGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALTSFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNRSARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIVGAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLASWREAS |
| Enzyme Length |
745 |
| Uniprot Accession Number |
P05164 |
| Absorption |
|
| Active Site |
ACT_SITE 261; /note=Proton acceptor |
| Activity Regulation |
|
| Binding Site |
BINDING 260; /note="Heme b; covalent, via 3 links"; BINDING 408; /note="Heme b; covalent, via 3 links"; BINDING 409; /note="Heme b; covalent, via 3 links" |
| Calcium Binding |
|
| catalytic Activity |
CATALYTIC ACTIVITY: Reaction=chloride + H(+) + H2O2 = H2O + hypochlorous acid; Xref=Rhea:RHEA:28218, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:17996, ChEBI:CHEBI:24757; EC=1.11.2.2; Evidence={ECO:0000269|PubMed:9922160}; |
| DNA Binding |
|
| EC Number |
1.11.2.2 |
| Enzyme Function |
FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971). {ECO:0000269|PubMed:25698971, ECO:0000269|PubMed:9922160}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Active site (1); Alternative sequence (2); Beta strand (20); Binding site (3); Chain (5); Disulfide bond (7); Glycosylation (6); Helix (28); Metal binding (6); Modified residue (1); Natural variant (8); Sequence conflict (2); Signal peptide (1); Site (1); Turn (10) |
| Keywords |
3D-structure;Alternative splicing;Calcium;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Lysosome;Metal-binding;Oxidation;Oxidoreductase;Peroxidase;Reference proteome;Signal |
| Interact With |
P27918; Q9UNE7 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Lysosome. |
| Modified Residue |
MOD_RES 316; /note=Cysteine sulfenic acid (-SOH); /evidence=ECO:0000269|PubMed:7840679 |
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..48; /evidence="ECO:0000269|PubMed:20332087, ECO:0000269|PubMed:2154223" |
| Structure 3D |
X-ray crystallography (19) |
| Cross Reference PDB |
1CXP;
1D2V;
1D5L;
1D7W;
1DNU;
1DNW;
1MHL;
1MYP;
3F9P;
3ZS0;
3ZS1;
4C1M;
4DL1;
4EJX;
5FIW;
5MFA;
5UZU;
6AZP;
6BMT;
|
| Mapped Pubmed ID |
10519157;
10698479;
10742562;
10777476;
11087769;
11147929;
11161635;
11179831;
11423389;
11434421;
11479475;
11774269;
11794452;
11811524;
11813987;
11840286;
11861298;
11895912;
11943609;
11956078;
11960308;
11981455;
11986950;
12027420;
12052532;
12057865;
12089442;
12111688;
12135010;
12164325;
12209361;
12355548;
12393699;
12397651;
12432558;
12460800;
12496042;
12496043;
12515618;
12565898;
12606047;
12694338;
12707270;
12729191;
12773517;
12782337;
12792137;
12800195;
12810834;
12818404;
12861032;
12915675;
12946561;
12950060;
12952835;
12969520;
1320128;
14580687;
14617020;
14626895;
14646692;
14668325;
14716779;
14729580;
14730210;
14761515;
14972011;
15006595;
15023809;
15031357;
15068388;
15159316;
15184258;
15196853;
15199549;
15203186;
15222689;
15255951;
15299090;
15304260;
15331175;
15350145;
15379210;
15492293;
15504976;
15507753;
15507754;
15507759;
15507760;
15507769;
15507770;
15507771;
15536330;
15538122;
15600254;
15661916;
15705913;
15714076;
15718477;
15734083;
15829318;
15850382;
15884126;
15885363;
15894800;
15901995;
15911707;
15967795;
15996936;
16084535;
16107886;
16157195;
16170238;
16195240;
16221222;
16267254;
16289072;
16297214;
16326702;
16385446;
16430221;
16476719;
16484808;
16497665;
16504169;
16543247;
16606792;
16641309;
16732739;
16758302;
16829688;
16844322;
16883063;
16901848;
16956579;
17017121;
17074761;
17119198;
17145829;
17149600;
17159117;
17161406;
17174955;
17178637;
17209550;
17259657;
17304047;
17337886;
17355643;
17381162;
17424838;
17428572;
17438335;
17440118;
17451207;
17479404;
17483704;
17521322;
17549373;
17582430;
17595772;
17601350;
17613595;
17650507;
17701359;
17704068;
17726014;
17767014;
17855424;
17869258;
17875305;
17885617;
17896805;
17922231;
17925548;
17980933;
18042261;
18177875;
18205184;
18214807;
18237195;
18258609;
18271379;
18273043;
18280811;
18296681;
18314009;
18322643;
18331758;
18340529;
18344624;
18359301;
18435917;
18440308;
18441517;
18447907;
18452102;
18466756;
18505072;
18563468;
18617697;
18632753;
18636124;
18642288;
18662591;
18676680;
18676870;
18778866;
18784359;
18836923;
18854777;
18930040;
18977241;
19012698;
19030913;
19031316;
19059911;
19081093;
19126404;
19129321;
19138806;
19140875;
19147137;
19162321;
19170196;
19201174;
19237222;
19255063;
19255975;
19322015;
19339270;
19345674;
19402211;
19410253;
19420105;
19423521;
19423540;
19479063;
19490915;
19505917;
19524564;
19538950;
19543083;
19544176;
19556446;
19578796;
19589271;
19593802;
19608745;
19614918;
19622717;
19625176;
19626395;
19641039;
19646343;
19651119;
19651670;
19692168;
19726691;
19729601;
19731237;
19735726;
19752340;
19758957;
19761988;
19788922;
19789190;
19814685;
19816870;
19822138;
19828014;
19857655;
19857676;
19877306;
19878569;
19897513;
19902075;
19913121;
19916937;
19963114;
19995403;
20007081;
20026093;
20032816;
20043647;
20080069;
20091863;
20136960;
20146436;
20158910;
20201999;
20228064;
20237496;
20305551;
20332596;
20360068;
20364045;
20364135;
20399194;
20406964;
20413430;
20418356;
20437850;
20438785;
20485444;
20536280;
20568015;
20592714;
20598694;
20628086;
20636397;
20644561;
20646231;
20651370;
20663217;
206633;
20673159;
20673504;
20677014;
20714866;
20732340;
20848159;
20933108;
20951227;
20974672;
20974816;
21126969;
21176345;
21204009;
21292302;
21297906;
21327394;
21331575;
21407237;
21461569;
21540635;
21657847;
21699462;
21739466;
21779191;
21792769;
21795425;
21827295;
21856025;
21880315;
21880720;
21884783;
21907168;
21917261;
21934609;
21950958;
21967851;
22014237;
22094928;
22097722;
22126566;
22140557;
22219194;
22264587;
22331023;
22348603;
22352991;
22427832;
22462773;
22465269;
22659527;
22703762;
22739978;
22802447;
22810585;
22902565;
22912422;
22949765;
22959522;
22985908;
23016839;
23059132;
23066164;
23100268;
23124356;
23167629;
23260186;
23268804;
23342893;
23364307;
23384293;
23436245;
23521574;
23532856;
23535901;
23537682;
23583798;
23620142;
23624305;
23666398;
23691142;
23707661;
23734221;
23746122;
23773345;
23832536;
23840365;
23843990;
23893381;
23902751;
23908111;
23943392;
23979979;
23991124;
24035742;
24036021;
24083736;
24194519;
24267244;
24267251;
24271323;
24291125;
24291281;
24330098;
24338120;
24340057;
24355864;
24385592;
24391750;
24436331;
24457336;
24530881;
24534704;
24567965;
24583458;
24591758;
24631066;
24704632;
24719228;
24748187;
24786234;
24851620;
24882572;
24915973;
24976018;
25002508;
25056433;
25141318;
25261597;
25394640;
25482861;
25530680;
25614581;
25637720;
25697357;
25701357;
25759391;
25770662;
25823564;
25922506;
26066543;
26132583;
26179062;
26229458;
26307104;
26319155;
26323935;
26386352;
26392121;
26431910;
26541603;
26567811;
26576922;
26697531;
26714302;
26797862;
26833773;
26841645;
26978734;
27013444;
27048452;
27129797;
27197583;
27579896;
27706591;
27752292;
27797335;
27821628;
27836774;
27884085;
27903408;
27928450;
28013526;
28058679;
28069011;
28131839;
28135312;
28167245;
28187981;
28260049;
28279572;
28328864;
28339364;
28348079;
28397206;
28466093;
28575531;
28602123;
28645072;
28682877;
28764808;
28808028;
28905321;
29041000;
29114094;
29212118;
29306105;
29306874;
29383971;
29453088;
29496995;
29524428;
29540402;
29577557;
29590135;
29614255;
29618370;
29631687;
29875311;
29937309;
29970677;
29975476;
30056589;
30207165;
30237127;
30266105;
30278820;
30300402;
3032796;
30343390;
30448568;
30456985;
30539859;
30626536;
30726210;
30938436;
31175983;
31236695;
31247619;
31341419;
31358527;
31531730;
31653520;
31754972;
31864385;
31991297;
32050431;
32277104;
32314165;
32383462;
32544357;
32674726;
32734361;
32758447;
32758448;
32794199;
32928076;
32962819;
33023023;
33027796;
33048471;
33091151;
33228505;
33285335;
33460291;
33569703;
33590771;
33609556;
33661166;
33864596;
33886672;
34324318;
34379628;
34571507;
34611229;
34737733;
34741558;
34746027;
8692836;
9918702;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
83,869 |
| Kinetics |
|
| Metal Binding |
METAL 262; /note=Calcium; METAL 334; /note=Calcium; METAL 336; /note=Calcium; via carbonyl oxygen; METAL 338; /note=Calcium; METAL 340; /note=Calcium; METAL 502; /note=Iron (heme b axial ligand) |
| Rhea ID |
RHEA:28218 |
| Cross Reference Brenda |
1.11.2.2; |