| IED ID | IndEnz0018000717 |
| Enzyme Type ID | peroxidase000717 |
| Protein Name |
Lignin-forming anionic peroxidase EC 1.11.1.7 |
| Gene Name | |
| Organism | Nicotiana sylvestris (Wood tobacco) (South American tobacco) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana sylvestris (Wood tobacco) (South American tobacco) |
| Enzyme Sequence | MNTPTQSFRAKAAIFSLLLLSCMQCHAQLSATFYDNTCPNALNTIRTSVRQAISSERRMAASLIRLHFHDCFVQGCDASILLDETPSIESEKTALPNLGSARGFGIIEDAKREVEKICPGVVSCADILTVAARDASAAVGGPSWTVKLGRRDSTTASKTLAETDLPGPFDPLNRLISSFASKGLSTRDMVALSGAHTIGQAQCFLFRDRIYSNGTDIDAGFASTRRRQCPQEGENGNLAPLDLVTPNQFDNNYFKNLIQKKGLLQSDQVLFNGGSTDNIVSEYSNSARAFSSDFAAAMIKMGDISPLSGQNGIIRKVCGSVN |
| Enzyme Length | 322 |
| Uniprot Accession Number | Q02200 |
| Absorption | |
| Active Site | ACT_SITE 69; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012" |
| Activity Regulation | |
| Binding Site | BINDING 166; /note=Substrate; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; |
| DNA Binding | |
| EC Number | 1.11.1.7 |
| Enzyme Function | FUNCTION: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.; FUNCTION: Plays an integral role in secondary cell wall biosynthesis by the polymerization of cinnamyl alcohols into lignin and by forming rigid cross-links between cellulose, pectin, hydroxy-proline-rich glycoproteins, and lignin. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (1); Chain (1); Disulfide bond (4); Glycosylation (1); Metal binding (10); Modified residue (1); Signal peptide (1); Site (1) |
| Keywords | Calcium;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00297}. |
| Modified Residue | MOD_RES 28; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,646 |
| Kinetics | |
| Metal Binding | METAL 70; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 73; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 75; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 77; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 79; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 196; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 197; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 242; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 245; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 250; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Rhea ID | RHEA:56136 |
| Cross Reference Brenda |