IED ID | IndEnz0018000758 |
Enzyme Type ID | peroxidase000758 |
Protein Name |
Alpha-dioxygenase 1 Ca-COX1 |
Gene Name | T459_06886 |
Organism | Capsicum annuum (Capsicum pepper) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Capsiceae Capsicum (peppers) Capsicum annuum (Capsicum pepper) |
Enzyme Sequence | MSFIMSSLRNLFLSPLRSFIQKDFHDAFDSMTLLDKLLFMMVHFVDKLILWHRLPVFLGLAYLAARRHLHQEYNLINVGRTPTGVRSNPEDYPYRTADGKYNDPFNEGAGSQFSFFGRNVMPVDQHDKLKKPDPMVVATKLLARKNFMDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELRAPEEVASECPLKSFKFYKSKKTPTGFYEIKTGYLNRRTSWWDGSAIYGSNTEALKKVRTFEDGKLKLSADGLLEQDENGNIISGDVRNPWAGLLALQALFIQEHNLVCDTLKKEYPKLEDEDLYRHARLVTSAVIAKVHTIDWTVELLKTDTLLAGMRANWYGLLGKKFKDTFGHVGGSSLGGFVGMKKPENHGVPYSLTEEFVSVYRMHQLLPDTLQLRNIDATPGPNKSLPLTNEIPMEDLIGGKGEENLSRIGYTKQMVSMGHQACGALELMNYPIWMRDLIPQDVDGNDRPDHIDLAALEIYRDRERSVARYNEFRRRMLQIPISKWEDLTDDEEAIKMLREVYGDDVEELDLLVGMSAEKKIKGFAISETAFFIFLIMASRRLEADKFFTSNYNEETYTKKGLEWVNTTESLKDVLDRHYPEMTGKWMNSNSAFSVWDSSPEPHNPIPIYFRVPQQ |
Enzyme Length | 643 |
Uniprot Accession Number | A0A2G3AC72 |
Absorption | |
Active Site | ACT_SITE 167; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00298 |
Activity Regulation | |
Binding Site | BINDING 172; /note=Heme b; /evidence=ECO:0000250|UniProtKB:Q9SGH6; BINDING 489; /note=Heme b; /evidence=ECO:0000250|UniProtKB:Q9SGH6; BINDING 493; /note=Heme b; /evidence=ECO:0000250|UniProtKB:Q9SGH6 |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | |
Enzyme Function | FUNCTION: Alpha-dioxygenase that catalyzes the primary oxygenation step of a variety of 14-20 carbon fatty acids, containing up to three unsaturated bonds, into their corresponding 2R-hydroperoxides (Probable). Involved in the production of oxylipins that function in cell signaling, wound healing, and protection from infection (Probable). The lipid-derived signaling pathway is involved in the initial response of hot pepper plants to pathogen infection (Probable). {ECO:0000305|PubMed:11807143}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Binding site (3); Chain (1); Metal binding (6); Sequence conflict (3); Site (1) |
Keywords | Calcium;Dioxygenase;Fatty acid biosynthesis;Fatty acid metabolism;Heme;Iron;Lipid biosynthesis;Lipid metabolism;Metal-binding;Oxidoreductase;Oxylipin biosynthesis;Peroxidase;Plant defense;Reference proteome |
Interact With | |
Induction | INDUCTION: Induced by infection with the bacterial pathogen Xanthomonas campestris pv glycine 8ra. {ECO:0000269|PubMed:11807143}. |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 74,007 |
Kinetics | |
Metal Binding | METAL 168; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q9SGH6; METAL 220; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q9SGH6; METAL 222; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q9SGH6; METAL 224; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q9SGH6; METAL 226; /note=Calcium; /evidence=ECO:0000250|UniProtKB:Q9SGH6; METAL 392; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00298 |
Rhea ID | |
Cross Reference Brenda |