| IED ID | IndEnz0018000787 |
| Enzyme Type ID | peroxidase000787 |
| Protein Name |
NADH oxidase NOXase EC 7.1.1.2 |
| Gene Name | nox EF_1586 |
| Organism | Enterococcus faecalis (strain ATCC 700802 / V583) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Enterococcaceae Enterococcus Enterococcus faecalis (Streptococcus faecalis) Enterococcus faecalis (strain ATCC 700802 / V583) |
| Enzyme Sequence | MKVVVVGCTHAGTSAVKSILANHPEAEVTVYERNDNISFLSCGIALYVGGVVKNAADLFYSNPEELASLGATVKMEHNVEEINVDDKTVTAKNLQTGATETVSYDKLVMTTGSWPIIPPIPGIDAENILLCKNYSQANVIIEKAKDAKRVVVVGGGYIGIELVEAFVESGKQVTLVDGLDRILNKYLDKPFTDVLEKELVDRGVNLALGENVQQFVADEQGKVAKVITPSQEFEADMVIMCVGFRPNTELLKDKVDMLPNGAIEVNEYMQTSNPDIFAAGDSAVVHYNPSQTKNYIPLATNAVRQGMLVGRNLTEQKLAYRGTQGTSGLYLFGWKIGSTGVTKESAKLNGLDVEATVFEDNYRPEFMPTTEKVLMELVYEKGTQRIVGGQLMSKYDITQSANTLSLAVQNKMTVEDLAISDFFFQPHFDRPWNYLNLLAQAALENM |
| Enzyme Length | 446 |
| Uniprot Accession Number | P37061 |
| Absorption | |
| Active Site | ACT_SITE 10; /note=Proton acceptor; /evidence=ECO:0000250; ACT_SITE 42; /note=Redox-active; /evidence=ECO:0000269|PubMed:8262333 |
| Activity Regulation | |
| Binding Site | BINDING 32; /note=FAD; /evidence=ECO:0000250; BINDING 42; /note=FAD; /evidence=ECO:0000250; BINDING 177; /note=NAD; /evidence=ECO:0000250; BINDING 186; /note=NAD; /evidence=ECO:0000250; BINDING 243; /note=NAD; via amide nitrogen; /evidence=ECO:0000250; BINDING 299; /note=FAD; via amide nitrogen; /evidence=ECO:0000250; BINDING 328; /note=NAD; via carbonyl oxygen; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2; |
| DNA Binding | |
| EC Number | 7.1.1.2 |
| Enzyme Function | FUNCTION: Catalyzes the four-electron reduction of molecular oxygen to water. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 7..11; /note=FAD; /evidence=ECO:0000250; NP_BIND 110..113; /note=FAD; /evidence=ECO:0000250; NP_BIND 150..165; /note=NAD; /evidence=ECO:0000250; NP_BIND 271..281; /note=FAD; /evidence=ECO:0000250 |
| Features | Active site (2); Binding site (7); Chain (1); Modified residue (1); Nucleotide binding (4) |
| Keywords | Direct protein sequencing;FAD;Flavoprotein;NAD;Oxidation;Oxidoreductase;Redox-active center;Reference proteome;Translocase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 42; /note=Cysteine sulfenic acid (-SOH); /evidence=ECO:0000269|PubMed:8262333 |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,915 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:29091 |
| Cross Reference Brenda |