Detail Information for IndEnz0018000802
IED ID IndEnz0018000802
Enzyme Type ID peroxidase000802
Protein Name Catalase-peroxidase
CP
EC 1.11.1.21
Peroxidase/catalase
Gene Name katG FTN_0633
Organism Francisella tularensis subsp. novicida (strain U112)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Thiotrichales Francisellaceae Francisella Francisella tularensis Francisella novicida Francisella tularensis subsp. novicida (strain U112)
Enzyme Sequence MLKKIVTALGMSGMLLASNSAIADDKNTETATPQSVDLSPLRNLNKLDSPMDKDYNYHQAFKKLDPEQLKKDMQDLLTQSQDWWPADFGNYGPFFIRLSWHDAGTYRIYDGRGGANRGQQRFSPLNSWPDNVNLDKARQLLWPIKQKYGDAVSWSDLIVLAGTVSLESMGMKPIGFAFGREDDWQGDDTNWGLSPEEIMSSNVRDGKLAPAYAATQMGLIYVNPEGPDGKPDIKGAASEIRQAFRAMGMTDKETVALIAGGHTFGKTHGAVPEDKVKEAIGPAPDKAPIEQQGLGWHNSYGTGNGDDTMGSGLEGSWTSTPTFWNHDFLHNLYNLNWKKTLSPAGAHQWTPTNAKPENMVPDAHKLGVKHKPIMFTTDLALKEDDGFNKYTQEFYNNPEEFKEEFAKAWFKLTHRDMGPKSRYIGPWIPEQNFIWQDPVPAADYKQVSTQDIAQLKQDIIDSGLTNQQLIKTAWDSASTYRKTDYRGGSNGARIALAPEKDWQMNEPAKLEVVLAKLKEIQTNFNNSKTDGTKVSLADLIVLGGNVGVEQAAKQAGYNIQIPFVPGRTDATQAQTDIESFNYLKTKSDGFINYTDGSVSADKLPQALVEKASMLDLNIPEMTVLVGGMRALNVNYDNSQEGVLTTTPGQLNNSFFVNLLDMSTQWKKSDKKDGEYIGIGRKTGKQKWTASPVDLIFGSNSELKAVAQVYAENGNEQKFVNDFAKAWHKVMMLGRFDVQQ
Enzyme Length 739
Uniprot Accession Number A0Q5L1
Absorption
Active Site ACT_SITE 101; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01961
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961};
DNA Binding
EC Number 1.11.1.21
Enzyme Function FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. {ECO:0000255|HAMAP-Rule:MF_01961}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Cross-link (2); Metal binding (1); Signal peptide (1); Site (1)
Keywords Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}.
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255|HAMAP-Rule:MF_01961
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 82,132
Kinetics
Metal Binding METAL 262; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_01961
Rhea ID RHEA:30275; RHEA:20309
Cross Reference Brenda