Detail Information for IndEnz0018000808
IED ID IndEnz0018000808
Enzyme Type ID peroxidase000808
Protein Name Hydroxylamine reductase
EC 1.7.99.1
Hybrid-cluster protein
HCP
Prismane protein
Gene Name hcp MJ0765
Organism Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic Lineage cellular organisms Archaea Euryarchaeota Methanomada group Methanococci Methanococcales Methanocaldococcaceae Methanocaldococcus Methanocaldococcus jannaschii Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Enzyme Sequence MFCFQCQEAAKNEGCTIKGVCGKDDVVANLQDLLIYTIKGLCYVCDKGNYLDDEVMDYIPKALFVTITNVNFDDKDVINWIKKGVALREKIIEKNNLNKEELPYCATWAYETDEDLINLANTKEVSVLAEDNEDIRSLKELITYGIKGIGAYLSHAMHLGYNNEDIHKFIIKAFTKIVDSKDADELFNLAMETGKYAVETLALLDKANTETYGHPEITEVNLGVRDRPGILISGHDLKDLEQLLEQSKDAGVDIYTHCEMLPAHYYPFFKKYEHFVGNYGGSWPFQREEFEKFNGPIVMTTNCLVPPKDSYKDRVYVTNEVGYPGLKRIPVKEDGTKDFSEVIEHAKKCKPPTPLENGKIVGGFAHNQVLALADKVIEAVKSGKIRKFVVMAGCDGRHKTREYYTEFAKKLPKDTVILTCGCAKYRFIKLDLGDIDGIPRVLDAGQCNDSYSLVKIALALKDAFGLNDVNELPIAYNISWYEQKAVTVLLALLYLGVKNIVLGPTLPAFLSPNVTKVLVEKFGISTISTVDEDIKRLVG
Enzyme Length 539
Uniprot Accession Number Q58175
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine; Xref=Rhea:RHEA:22052, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15429, ChEBI:CHEBI:17499, ChEBI:CHEBI:28938; EC=1.7.99.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00069};
DNA Binding
EC Number 1.7.99.1
Enzyme Function FUNCTION: Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O. {ECO:0000255|HAMAP-Rule:MF_00069}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Erroneous initiation (1); Metal binding (12); Modified residue (1)
Keywords 4Fe-4S;Cytoplasm;Iron;Iron-sulfur;Metal-binding;Oxidoreductase;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00069}.
Modified Residue MOD_RES 394; /note=Cysteine persulfide; /evidence=ECO:0000255|HAMAP-Rule:MF_00069
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 60,458
Kinetics
Metal Binding METAL 3; /note=Iron-sulfur (4Fe-4S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 6; /note=Iron-sulfur (4Fe-4S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 15; /note=Iron-sulfur (4Fe-4S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 21; /note=Iron-sulfur (4Fe-4S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 235; /note=Iron-oxo-sulfur (4Fe-2O-2S); via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 259; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 303; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 394; /note=Iron-oxo-sulfur (4Fe-2O-2S); via persulfide group; /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 422; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 447; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 482; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069; METAL 484; /note=Iron-oxo-sulfur (4Fe-2O-2S); /evidence=ECO:0000255|HAMAP-Rule:MF_00069
Rhea ID RHEA:22052
Cross Reference Brenda