| IED ID | IndEnz0018000941 |
| Enzyme Type ID | peroxidase000941 |
| Protein Name |
L-ascorbate peroxidase S, chloroplastic/mitochondrial EC 1.11.1.11 Stromal ascorbate peroxidase AtAPx05 sAPX |
| Gene Name | APXS At4g08390 T28D5.80 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAERVSLTLNGTLLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSSSRSTLTLSASSSLSFVRSLVSSPRLSSSSSLSQKKYRIASVNRSFNSTTAATKSSSSDPDQLKNAREDIKELLSTKFCHPILVRLGWHDAGTYNKNIKEWPQRGGANGSLRFDIELKHAANAGLVNALNLIKDIKEKYSGISYADLFQLASATAIEEAGGPKIPMKYGRVDASGPEDCPEEGRLPDAGPPSPATHLREVFYRMGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI |
| Enzyme Length | 372 |
| Uniprot Accession Number | Q42592 |
| Absorption | |
| Active Site | ACT_SITE 133; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Metal binding (4); Region (3); Sequence conflict (4); Site (1); Transit peptide (1) |
| Keywords | Alternative splicing;Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Metal-binding;Mitochondrion;Oxidoreductase;Peroxidase;Plastid;Potassium;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:12954611, ECO:0000305|PubMed:25732537}. Plastid, chloroplast stroma {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11997377; 12068123; 12177459; 14671022; 17432890; 17823777; 18318659; 18431481; 18633119; 19284649; 19859717; 20007290; 20118269; 20584316; 21895668; 22131159; 23162559; 24064926; 24203232; 25482793; 26292995; 27014325; 27247031; 27439459; 28627464; 28835225; 29576081; 31257201; 32564178; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,407 |
| Kinetics | |
| Metal Binding | METAL 262; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 263; /note=Potassium; /evidence=ECO:0000250; METAL 295; /note=Potassium; /evidence=ECO:0000250; METAL 302; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda | 1.11.1.11; |