| IED ID | IndEnz0018000942 |
| Enzyme Type ID | peroxidase000942 |
| Protein Name |
Probable L-ascorbate peroxidase 7, chloroplastic EC 1.11.1.11 OsAPx7 |
| Gene Name | APX7 Os04g0434800 LOC_Os04g35520 OsJ_14879 OSJNBb0086G13.10 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MAAQRLAALHAAAPSAFSSTSSASHGRPAARSSTTALLPVALPRASATLRAAPSRLLPQEAKAAGSGRSVMCMASASASAASAAVASGAAELKAAREDIRELLKTTHCHPILVRLGWHDSGTYDKNIKEWPQRGGANGSLRFDVELKHGANAGLVNALKLVQPIKDKYPNISYADLFQLASATAIEEAGGPKIPMTYGRIDVTGPEQCPPEGKLPDAGPSAPADHLRKVFYRMGLDDKEIVVLSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGGQSWTAEWLKFDNSYFKEIKEKRDQDLLVLPTDAALFEDPTFKVYAEKYAEDQEAFFKDYAGAHAKLSNLGAKFNPPEGFTLDG |
| Enzyme Length | 359 |
| Uniprot Accession Number | Q7XJ02 |
| Absorption | |
| Active Site | ACT_SITE 118; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (1); Metal binding (4); Region (1); Site (1); Transit peptide (1) |
| Keywords | Calcium;Chloroplast;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Plastid;Potassium;Reference proteome;Transit peptide |
| Interact With | |
| Induction | INDUCTION: Induced by salt stress (PubMed:16397796). Down-regulated by hydrogen peroxide in leaves (PubMed:25546583). {ECO:0000269|PubMed:16397796, ECO:0000269|PubMed:25546583}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17916638; 19704658; 24268165; 24280096; |
| Motif | |
| Gene Encoded By | |
| Mass | 38,325 |
| Kinetics | |
| Metal Binding | METAL 247; /note=Iron (heme b axial ligand); METAL 248; /note=Potassium; /evidence=ECO:0000250; METAL 280; /note=Potassium; /evidence=ECO:0000250; METAL 287; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda | 1.11.1.11; |