| IED ID | IndEnz0018000998 |
| Enzyme Type ID | peroxidase000998 |
| Protein Name |
L-ascorbate peroxidase, cytosolic AP EC 1.11.1.11 PsAPx01 |
| Gene Name | APX1 APPX1 |
| Organism | Pisum sativum (Garden pea) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina IRL clade Fabeae Pisum Pisum sativum (Garden pea) |
| Enzyme Sequence | MGKSYPTVSPDYQKAIEKAKRKLRGFIAEKKCAPLILRLAWHSAGTFDSKTKTGGPFGTIKHQAELAHGANNGLDIAVRLLEPIKEQFPIVSYADFYQLAGVVAVEITGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLTGEKDGLLQLPSDKALLTDSVFRPLVEKYAADEDVFFADYAEAHLKLSELGFAEA |
| Enzyme Length | 250 |
| Uniprot Accession Number | P48534 |
| Absorption | |
| Active Site | ACT_SITE 42; /note=Proton acceptor |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (2); Chain (1); Compositional bias (1); Helix (14); Initiator methionine (1); Metal binding (6); Region (1); Site (1); Turn (5) |
| Keywords | 3D-structure;Calcium;Cytoplasm;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Potassium;Stress response |
| Interact With | |
| Induction | INDUCTION: By stress. {ECO:0000269|PubMed:1400489}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1APX; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,193 |
| Kinetics | |
| Metal Binding | METAL 163; /note="Iron (heme b axial ligand)"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000269|PubMed:7703247"; METAL 164; /note="Potassium"; /evidence="ECO:0000269|PubMed:7703247, ECO:0007744|PDB:1APX"; METAL 180; /note="Potassium"; /evidence="ECO:0000269|PubMed:7703247, ECO:0007744|PDB:1APX"; METAL 182; /note="Potassium"; /evidence="ECO:0000269|PubMed:7703247, ECO:0007744|PDB:1APX"; METAL 185; /note="Potassium; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:7703247, ECO:0007744|PDB:1APX"; METAL 187; /note="Potassium"; /evidence="ECO:0000269|PubMed:7703247, ECO:0007744|PDB:1APX" |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda |