| IED ID | IndEnz0018001006 |
| Enzyme Type ID | peroxidase001006 |
| Protein Name |
Non-heme chloroperoxidase CPO-A1 CPO-A1 EC 1.11.1.- BPO1 Bromide peroxidase Non-haem bromoperoxidase BPO-A1 |
| Gene Name | bpoA1 |
| Organism | Kitasatospora aureofaciens (Streptomyces aureofaciens) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Kitasatospora Kitasatospora aureofaciens (Streptomyces aureofaciens) |
| Enzyme Sequence | MPICTTRDGVEIFYKDWGQGRPVVFIHGWPLNGDAWQDQLKAVVDAGYRGIAHDRRGHGHSTPVWDGYDFDTFADDLNDLLTDLDLRDVTLVAHSMGGGELARYVGRHGTGRLRSAVLLSAIPPVMIKSDKNPDGVPDEVFDALKNGVLTERSQFWKDTAEGFFSANRPGNKVTQGNKDAFWYMAMAQTIEGGVRCVDAFGYTDFTEDLKKFDIPTLVVHGDDDQVVPIDATGRKSAQIIPNAELKVYEGSSHGIAMVPGDKEKFNRDLLEFLNK |
| Enzyme Length | 275 |
| Uniprot Accession Number | P33912 |
| Absorption | |
| Active Site | ACT_SITE 95; /note=Charge relay system; /evidence=ECO:0000305; ACT_SITE 224; /note=Charge relay system; /evidence=ECO:0000305; ACT_SITE 253; /note=Charge relay system; /evidence=ECO:0000305 |
| Activity Regulation | ACTIVITY REGULATION: Brominating activity not inhibited by azide, peroxidase activity stimulated by bromide. {ECO:0000269|PubMed:1783900}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 1.11.1.- |
| Enzyme Function | FUNCTION: May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis. Able to brominate as well. {ECO:0000269|PubMed:1783900}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (9); Chain (1); Domain (1); Helix (11); Initiator methionine (1); Sequence conflict (2); Turn (2) |
| Keywords | 3D-structure;Antibiotic biosynthesis;Direct protein sequencing;Oxidoreductase;Peroxidase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1A8Q; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 30,475 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |