| IED ID | IndEnz0018001018 |
| Enzyme Type ID | peroxidase001018 |
| Protein Name |
L-ascorbate peroxidase 3 AtAPx03 EC 1.11.1.11 |
| Gene Name | APX3 APX APXIII At4g35000 M4E13.60 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAAPIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNEEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSNVCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPLKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELGFNPNSSAGKAVADSTILAQSAFGVAVAAAVVAFGYFYEIRKRMK |
| Enzyme Length | 287 |
| Uniprot Accession Number | Q42564 |
| Absorption | |
| Active Site | ACT_SITE 40; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O2 + L-ascorbate = 2 H2O + L-dehydroascorbate; Xref=Rhea:RHEA:22996, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:38290, ChEBI:CHEBI:58539; EC=1.11.1.11; |
| DNA Binding | |
| EC Number | 1.11.1.11 |
| Enzyme Function | FUNCTION: Plays a key role in hydrogen peroxide removal. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Initiator methionine (1); Metal binding (4); Modified residue (1); Motif (1); Region (1); Sequence conflict (3); Site (1); Transmembrane (1) |
| Keywords | Acetylation;Calcium;Glyoxysome;Heme;Hydrogen peroxide;Iron;Membrane;Metal-binding;Oxidoreductase;Peroxidase;Peroxisome;Potassium;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000269|PubMed:16873450, ECO:0000269|PubMed:20215589}; Single-pass membrane protein {ECO:0000269|PubMed:16873450}. Glyoxysome membrane {ECO:0000269|PubMed:16873450}; Single-pass membrane protein {ECO:0000269|PubMed:16873450}. |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22223895 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10501032; 11483357; 11862948; 12068123; 12225588; 12766230; 12789421; 12805617; 12938931; 12954611; 14581625; 14671017; 14701923; 14760709; 15276431; 15276449; 15308753; 15608336; 15695592; 16006522; 16034597; 16336779; 16531488; 17151019; 17449810; 17660356; 18315867; 18431481; 18775970; 18931141; 21166475; 22034551; 22789293; 24064926; 28627464; 31257201; |
| Motif | MOTIF 281..287; /note=AKR2A-binding sequence (ABS) required for peroxisome membrane targeting; /evidence=ECO:0000269|PubMed:20215589 |
| Gene Encoded By | |
| Mass | 31,572 |
| Kinetics | |
| Metal Binding | METAL 160; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 161; /note=Potassium; /evidence=ECO:0000250; METAL 177; /note=Potassium; /evidence=ECO:0000250; METAL 184; /note=Potassium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:22996 |
| Cross Reference Brenda |