| IED ID | IndEnz0018001053 |
| Enzyme Type ID | peroxidase001053 |
| Protein Name |
Catalase-peroxidase 2 CP 2 EC 1.11.1.21 Peroxidase/catalase 2 |
| Gene Name | katG2 katH MSMEG_3461 MSMEI_3380 |
| Organism | Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycolicibacterium Mycolicibacterium smegmatis (Mycobacterium smegmatis) Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) |
| Enzyme Sequence | MSSDTSDSRPPNPDTKTASTSESENPAIPSPKPKSGAPLRNQDWWPNQIDVSRLHPHPPQGNPLGEDFDYAEEFAKLDVNALKADLTALMTQSQDWWPADYGHYGGLFIRMSWHSAGTYRIHDGRGGGGQGAQRFAPINSWPDNVSLDKARRLLWPIKQKYGNKISWADLLVFTGNVALESMGFKTFGFGFGREDIWEPEEILFGEEDEWLGTDKRYGGGEQRQLAEPYGATTMGLIYVNPEGPEGQPDPLAAAHDIRETFGRMAMNDEETAALIVGGHTFGKTHGAGDASLVGPEPEAAPIEQQGLGWKSSYGTGKGPDTITSGLEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGAWQFTAKDGAGAGTIPDPFGGPGRNPTMLVTDISMRVDPIYGKITRRWLDHPEELSEAFAKAWYKLLHRDMGPISRYLGPWVAEPQLWQDPVPAVDHPLVDDQDIAALKSTVLDSGLSTGQLIKTAWASAASYRNTDKRGGANGARVRLEPQKNWDVNEPAELATVLPVLERIQQDFNASASGGKKVSLADLIVLAGSAAIEKAAKDGGYNVTVPFAPGRTDASQENTDVESFAVLEPRADGFRNYVRPGEKVQLEKMLLERAYFLGVTAPQLTALVGGLRALDVNHGGTKHGVFTDRPGALTNDFFVNLLDMGTEWKTSETTENVYEGVDRKTGQLKWTATANDLVFGSHSVLRAVAEVYAQSDNGERFVNDFVKAWVKVMNNDRFDLK |
| Enzyme Length | 748 |
| Uniprot Accession Number | A0QXX7 |
| Absorption | |
| Active Site | ACT_SITE 114; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Cross-link (2); Initiator methionine (1); Metal binding (1); Region (1); Site (1) |
| Keywords | Direct protein sequencing;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 81,998 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.4 mM for H(2)O(2) for the catalase reaction {ECO:0000269|PubMed:7673210}; KM=0.19 mM for o-dianisidine for the peroxidase reaction {ECO:0000269|PubMed:7673210}; KM=0.17 mM for NADPH for the peroxidase reaction {ECO:0000269|PubMed:7673210}; KM=0.23 mM for NADH for the peroxidase reaction {ECO:0000269|PubMed:7673210}; KM=0.31 mM for pyrogallol for the peroxidase reaction {ECO:0000269|PubMed:7673210}; KM=0.11 mM for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) for the peroxidase reaction {ECO:0000269|PubMed:7673210}; |
| Metal Binding | METAL 279; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda |