| IED ID | IndEnz0018001064 |
| Enzyme Type ID | peroxidase001064 |
| Protein Name |
Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic PHGPx EC 1.11.1.12 |
| Gene Name | GPX1 At2g25080 F13D4.40 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVSMTTSSSSYGTFSTVVNSSRPNSSATFLVPSLKFSTGISNFANLSNGFSLKSPINPGFLFKSRPFTVQARAAAEKTVHDFTVKDIDGKDVALNKFKGKVMLIVNVASRCGLTSSNYSELSHLYEKYKTQGFEILAFPCNQFGFQEPGSNSEIKQFACTRFKAEFPIFDKVDVNGPSTAPIYEFLKSNAGGFLGGLIKWNFEKFLIDKKGKVVERYPPTTSPFQIEKDIQKLLAA |
| Enzyme Length | 236 |
| Uniprot Accession Number | P52032 |
| Absorption | |
| Active Site | ACT_SITE 111; /evidence=ECO:0000250|UniProtKB:P36968 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a hydroperoxy polyunsaturated fatty acid + 2 glutathione = a hydroxy polyunsaturated fatty acid + glutathione disulfide + H2O; Xref=Rhea:RHEA:19057, ChEBI:CHEBI:15377, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297, ChEBI:CHEBI:131871, ChEBI:CHEBI:134019; EC=1.11.1.12; Evidence={ECO:0000250|UniProtKB:P36968}; |
| DNA Binding | |
| EC Number | 1.11.1.12 |
| Enzyme Function | FUNCTION: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. {ECO:0000250|UniProtKB:O70325}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Region (1); Sequence conflict (2); Transit peptide (1) |
| Keywords | Chloroplast;Oxidoreductase;Peroxidase;Plastid;Reference proteome;Stress response;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By salt stress, osmotic stress, metals and heat treatment. Up-regulated by abscisic acid (ABA) and auxin. {ECO:0000269|PubMed:14617062}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11027730; 12609038; 12766230; 12885779; 12913160; 12938931; 12953064; 14576160; 15028209; 15053760; 15322131; 15829605; 15890615; 16856986; 16913859; 17096689; 17823777; 18218973; 18230142; 18431481; 18493039; 18633119; 19363092; 20584316; 22339086; 23661340; 24470466; 27014325; 27247031; 27676073; 28102911; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 26,016 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:19057 |
| Cross Reference Brenda |