| IED ID | IndEnz0018001076 |
| Enzyme Type ID | peroxidase001076 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Peroxidase/catalase |
| Gene Name | katG AO090010000722 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MADKCPFHNQAPKPNVAGSGTQNRDWWPDQLKLNILRQHTTVSNPLDPDFDYAAAFNSLDYYALKKDLQDLMTDSQDWWPADFGHYGGLFIRMAWHSAGTYRTFDGRGGGGQGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGNKISWADLMILTGNVALESMGFKTFGFAGGRKDTWEADESVYWGGETTWLGNDVRYSHGFAGSSKHGAVIADEASHRDIHSRELEKPLAAAHMGLIYVNPEGPDGNPDPVAAARDIRTTFARMAMNDEETVALIAGGHTFGKTHGAASSDHVGSEPEAAGLEAQGLGWQNSHGSGKGAHTITSGLEVTWTKTPTQWNLNFLEYLFRFEWVLTKSPAGANQWVAKDADAFIPDAYDSSKKHRPQMLTTDLSLRFDPAYEKISRRFLENPDQFAEAFARAWFKLTHRDMGPRARYIGPEVPAEELSWQDPIPAVNHPVISETDIAALKRDILATGVDPSKFISTAWASASTFRGSDKRGGANGARIRLAPQRDWEVNNQPWLAAALKALEDIQDKFNSAQNDGKRVSLADLIVLAGCAAVEKAASDAGHIITVPFTPGRMDASQDQTDVESFNQMEPVADGFRNYGTSTARVPAEHYLVDKAQLLTLSAPEMTVLVGGLRALNANYDGSAHGVFTTRPGQLTNDFFVNLLDMNTSWKASGSGNDIYEGTDRRTGSKKWTATRADLVFGSHAELRAIAEVYGSSDGKGKFVKDFVAAWAKVMNLDRFDVN |
| Enzyme Length | 751 |
| Uniprot Accession Number | Q2TW34 |
| Absorption | |
| Active Site | ACT_SITE 96; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_03108 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_03108}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. {ECO:0000255|HAMAP-Rule:MF_03108}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Cross-link (2); Metal binding (1); Region (1); Site (1) |
| Keywords | Cytoplasm;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03108}. |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_03108}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 82,628 |
| Kinetics | |
| Metal Binding | METAL 282; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_03108 |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda |