| IED ID | IndEnz0018001085 |
| Enzyme Type ID | peroxidase001085 |
| Protein Name |
Catalase-peroxidase CP EC 1.11.1.21 Peroxidase/catalase |
| Gene Name | katG BURPS1710b_3366 |
| Organism | Burkholderia pseudomallei (strain 1710b) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Burkholderia pseudomallei group Burkholderia pseudomallei (Pseudomonas pseudomallei) Burkholderia pseudomallei (strain 1710b) |
| Enzyme Sequence | MSNEAKCPFHQAAGNGTSNRDWWPNQLDLSILHRHSSLSDPMGKDFNYAQAFEKLDLAAVKRDLHALMTTSQDWWPADFGHYGGLFIRMAWHSAGTYRTADGRGGAGEGQQRFAPLNSWPDNANLDKARRLLWPIKQKYGRAISWADLLILTGNVALESMGFKTFGFAGGRADTWEPEDVYWGSEKIWLELSGGPNSRYSGDRQLENPLAAVQMGLIYVNPEGPDGNPDPVAAARDIRDTFARMAMNDEETVALIAGGHTFGKTHGAGPASNVGAEPEAAGIEAQGLGWKSAYRTGKGADAITSGLEVTWTTTPTQWSHNFFENLFGYEWELTKSPAGAHQWVAKGADAVIPDAFDPSKKHRPTMLTTDLSLRFDPAYEKISRRFHENPEQFADAFARAWFKLTHRDMGPRARYLGPEVPAEVLLWQDPIPAVDHPLIDAADAAELKAKVLASGLTVSQLVSTAWAAASTFRGSDKRGGANGARIRLAPQKDWEANQPEQLAAVLETLEAIRTAFNGAQRGGKQVSLADLIVLAGCAGVEQAAKNAGHAVTVPFAPGRADASQEQTDVESMAVLEPVADGFRNYLKGKYRVPAEVLLVDKAQLLTLSAPEMTVLLGGLRVLGANVGQSRHGVFTAREQALTNDFFVNLLDMGTEWKPTAADADVFEGRDRATGELKWTGTRVDLVFGSHSQLRALAEVYGSADAQEKFVRDFVAVWNKVMNLDRFDLA |
| Enzyme Length | 728 |
| Uniprot Accession Number | Q3JNW6 |
| Absorption | |
| Active Site | ACT_SITE 92; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=AH2 + H2O2 = A + 2 H2O; Xref=Rhea:RHEA:30275, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17499; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; CATALYTIC ACTIVITY: Reaction=2 H2O2 = 2 H2O + O2; Xref=Rhea:RHEA:20309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240; EC=1.11.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01961}; |
| DNA Binding | |
| EC Number | 1.11.1.21 |
| Enzyme Function | FUNCTION: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (17); Chain (1); Cross-link (2); Helix (47); Metal binding (1); Site (1); Turn (7) |
| Keywords | 3D-structure;Heme;Hydrogen peroxide;Iron;Metal-binding;Oxidoreductase;Peroxidase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme. {ECO:0000255|HAMAP-Rule:MF_01961}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (49) |
| Cross Reference PDB | 5KQ0; 5KQ2; 5KQ3; 5KQ6; 5KQH; 5KQI; 5KQK; 5KQN; 5KQQ; 5KSF; 5KSG; 5KSK; 5KSN; 5KT8; 5KT9; 5L02; 5L05; 5SW4; 5SW5; 5SW6; 5SX0; 5SX1; 5SX2; 5SX3; 5SX6; 5SX7; 5SXQ; 5SXR; 5SXS; 5SXT; 5SXW; 5SXX; 5SYH; 5SYI; 5SYJ; 5SYK; 5SYL; 5SYU; 5SYV; 5SYW; 5SYY; 5TXQ; 5V4O; 5V53; 6B9B; 6CAW; 6CC6; 6CDQ; 6CEK; |
| Mapped Pubmed ID | 12628252; 15567407; 16211084; 16618106; 17063492; 20554537; 24568093; 24785434; 28409923; 29732452; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,446 |
| Kinetics | |
| Metal Binding | METAL 259; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|HAMAP-Rule:MF_01961 |
| Rhea ID | RHEA:30275; RHEA:20309 |
| Cross Reference Brenda | 1.11.1.21; |