IED ID | IndEnz0018001123 |
Enzyme Type ID | peroxidase001123 |
Protein Name |
Type 1 encapsulin shell protein IMEF encapsulin |
Gene Name | enc QY95_01592 |
Organism | Quasibacillus thermotolerans |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Quasibacillus Quasibacillus thermotolerans |
Enzyme Sequence | MNKSQLYPDSPLTDQDFNQLDQTVIEAARRQLVGRRFIELYGPLGRGMQSVFNDIFMESHEAKMDFQGSFDTEVESSRRVNYTIPMLYKDFVLYWRDLEQSKALDIPIDFSVAANAARDVAFLEDQMIFHGSKEFDIPGLMNVKGRLTHLIGNWYESGNAFQDIVEARNKLLEMNHNGPYALVLSPELYSLLHRVHKDTNVLEIEHVRELITAGVFQSPVLKGKSGVIVNTGRNNLDLAISEDFETAYLGEEGMNHPFRVYETVVLRIKRPAAICTLIDPEE |
Enzyme Length | 282 |
Uniprot Accession Number | A0A0F5HPP7 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | |
Enzyme Function | FUNCTION: Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous icosahedral shells 42-43 nm in diameter with an iron- and phosphorus-rich core (1Fe:1.1P) which can store over 23,000-35,000 iron atoms (with a calculated maximum of 83,000 Fe). There are 2 types of negatively charged open pores in the cryo-electron structure; a 3-fold pore where 3 hexamers meet with a minimal size of 7.2 Angstroms and a 5-fold pore where pentamers meet with a minimal size of 2.3 Angstroms. The 2-fold pore seen in other encapsulin nanocompartments is closed. Empty compartments can be generated in E.coli (PubMed:28263314, PubMed:31282860, PubMed:31194509). Both types of pore have extra density in their centers in the structure (PubMed:31282860). 2 different cargo proteins have been identified (IMEF and Fer); when both are expressed in E.coli with the shell protein only IMEF is detected within the nanocompartment. E.coli expressing all 3 genes stores the largest amount of iron and is protected from Fe/H2O2-induced oxidative stress (PubMed:28263314). Part of the iron-mineralizing encapsulin-associated Firmicute (IMEF) system (Probable). {ECO:0000269|PubMed:28263314, ECO:0000269|PubMed:31194509, ECO:0000269|PubMed:31282860, ECO:0000305|PubMed:28263314}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: The empty encapsulin nanocompartment is stable until 86.6 degrees Celsius, when loaded with cargo protein is stable until 88.9 degrees Celsius and when grown in high-iron conditions is stable until 91.8 degrees Celsius. {ECO:0000269|PubMed:31282860}; |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Site (5) |
Keywords | 3D-structure;Encapsulin nanocompartment;Ion transport;Iron;Iron storage;Iron transport;Reference proteome;Transport |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Encapsulin nanocompartment {ECO:0000269|PubMed:31194509, ECO:0000269|PubMed:31282860}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | Electron microscopy (1) |
Cross Reference PDB | 6NJ8; |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 32,204 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |