Detail Information for IndEnz0018001124
IED ID IndEnz0018001124
Enzyme Type ID peroxidase001124
Protein Name Dye-decolorizing peroxidase
MepDyP
EC 1.11.1.19
EC 1.11.1.7
Gene Name dyp1
Organism Mycena epipterygia (Yellow-stemmed mycena)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Mycenaceae Mycena Mycena epipterygia (Yellow-stemmed mycena)
Enzyme Sequence MRKSISTFILLSVLSVGQLVAARPRSTNAPPRRRTPQPRRTTSLFINPPALPDLPTVQAVDKLDDALDIDNIQSDILVGMKKTKELFFFFGVEDAATFKSKLASDILPLITTTTEILSTDTQPITAVNIAFSHTGLVALGVNDDIGDTDFVSGQFSEATTVGDRPTLWDPAFAGTKIHGVFLLASNTTEDINTELANIQSILGSSITEIHRLQGAARPGAEEGHEHFGFLDGISQPAVNGFTDTVLPGQTPVDPGLFLLGESGDPSQDSRPSWAVDGSFLVFRQLQQFVPEFNQFLADHPLDIPGRTAEEGSELLGARMVGRWKSGAPVFLSPTQDDPVLGADKNRNNDFNYLVASDPDPGTENHFNQTACPFGAHIRKVHPRGDLSPSAENPHFIIRSSIPYGPEVTDAEAASSTTSVDRGLAFVSYQSNIFNGFVFLQSIWINNVNFIFGKVDPTIGVDPIIGTLGGGPHNISGLNPLIPVNETVDFQDDPWNDVVIPRDFVVSHGGEYFFSPSLSGISAIAAA
Enzyme Length 526
Uniprot Accession Number I2DBY3
Absorption BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Abs(max)=405 nm {ECO:0000269|PubMed:23111597};
Active Site ACT_SITE 231; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:I2DBY1
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 H2O2 + Reactive Blue 5 = 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate + 2 H(+) + 2 H2O + phthalate; Xref=Rhea:RHEA:28086, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:17563, ChEBI:CHEBI:63950, ChEBI:CHEBI:63955, ChEBI:CHEBI:64278; EC=1.11.1.19; Evidence={ECO:0000269|PubMed:23111597}; CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; Evidence={ECO:0000269|PubMed:23111597};
DNA Binding
EC Number 1.11.1.19; 1.11.1.7
Enzyme Function FUNCTION: Manganese-independent peroxidase that is able to convert a large number of compounds, but its physiological substrate is not known. In addition to classic peroxidase substrates (e.g. 2,6-dimethoxyphenol), oxidizes dyes such as Reactive Blue 5 and Reactive Black 5. {ECO:0000269|PubMed:23111597}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable. Retains 50%-90% of its activity after heating for 2 hours at 60 degrees Celsius, while no decrease in activity is observed within the same time at 30 or 40 degrees Celsius. {ECO:0000269|PubMed:23111597};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4 with 2,6-dimethoxyphenol as substrate. Retains 90% of its activity after 4 hours at pH 2.5. {ECO:0000269|PubMed:23111597};
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Glycosylation (4); Metal binding (1); Propeptide (1); Signal peptide (1)
Keywords Direct protein sequencing;Glycoprotein;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:23111597}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 56,644
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=11 uM for H(2)O(2) {ECO:0000269|PubMed:23111597};
Metal Binding METAL 376; /note=Iron (heme axial ligand); via tele nitrogen; /evidence=ECO:0000250|UniProtKB:I2DBY1
Rhea ID RHEA:28086; RHEA:56136
Cross Reference Brenda