IED ID |
IndEnz0018001127 |
Enzyme Type ID |
peroxidase001127 |
Protein Name |
Hemoglobin subunit beta
Beta-globin
Hemoglobin beta chain
Cleaved into: LVV-hemorphin-7; Spinorphin
|
Gene Name |
HBB |
Organism |
Homo sapiens (Human) |
Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Primates
Haplorrhini
Simiiformes
Catarrhini
Hominoidea (apes)
Hominidae (great apes)
Homininae
Homo
Homo sapiens (Human)
|
Enzyme Sequence |
MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH |
Enzyme Length |
147 |
Uniprot Accession Number |
P68871 |
Absorption |
|
Active Site |
|
Activity Regulation |
|
Binding Site |
BINDING 2; /note="2,3-bisphosphoglycerate; via amino nitrogen"; BINDING 3; /note="2,3-bisphosphoglycerate"; BINDING 83; /note="2,3-bisphosphoglycerate"; BINDING 144; /note="2,3-bisphosphoglycerate" |
Calcium Binding |
|
catalytic Activity |
|
DNA Binding |
|
EC Number |
|
Enzyme Function |
FUNCTION: Involved in oxygen transport from the lung to the various peripheral tissues. {ECO:0000269|PubMed:28066926}.; FUNCTION: LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.; FUNCTION: [Spinorphin]: Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. |
Temperature Dependency |
|
PH Dependency |
|
Pathway |
|
nucleotide Binding |
|
Features |
Binding site (4); Chain (1); Glycosylation (6); Helix (11); Initiator methionine (1); Metal binding (2); Modified residue (11); Natural variant (261); Peptide (2); Sequence conflict (2); Site (22); Turn (1) |
Keywords |
3D-structure;Acetylation;Congenital dyserythropoietic anemia;Direct protein sequencing;Disease variant;Glycation;Glycoprotein;Heme;Hereditary hemolytic anemia;Hypotensive agent;Iron;Metal-binding;Oxygen transport;Phosphoprotein;Pyruvate;Reference proteome;S-nitrosylation;Transport;Vasoactive |
Interact With |
P69905; Q6B0K9; P09105; P02008 |
Induction |
|
Subcellular Location |
|
Modified Residue |
MOD_RES 2; /note="N-acetylvaline"; /evidence="ECO:0000250|UniProtKB:P02086"; MOD_RES 2; /note="N-pyruvate 2-iminyl-valine; in Hb A1b"; MOD_RES 10; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 13; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 45; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 51; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 60; /note="N6-acetyllysine"; /evidence="ECO:0000269|PubMed:4531009"; MOD_RES 83; /note="N6-acetyllysine"; /evidence="ECO:0000269|PubMed:4531009"; MOD_RES 88; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 94; /note="S-nitrosocysteine"; /evidence="ECO:0000269|PubMed:8637569, ECO:0000269|PubMed:9843411"; MOD_RES 145; /note="N6-acetyllysine; alternate"; /evidence="ECO:0000269|PubMed:4531009" |
Post Translational Modification |
PTM: Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.; PTM: S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2). {ECO:0000269|PubMed:1520632, ECO:0000269|PubMed:8637569, ECO:0000269|PubMed:9843411}.; PTM: Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. {ECO:0000269|PubMed:4531009}. |
Signal Peptide |
|
Structure 3D |
X-ray crystallography (285); Neutron diffraction (2); Electron microscopy (4); NMR spectroscopy (2) |
Cross Reference PDB |
1A00;
1A01;
1A0U;
1A0Z;
1A3N;
1A3O;
1ABW;
1ABY;
1AJ9;
1B86;
1BAB;
1BBB;
1BIJ;
1BUW;
1BZ0;
1BZ1;
1BZZ;
1C7B;
1C7C;
1C7D;
1CBL;
1CBM;
1CH4;
1CLS;
1CMY;
1COH;
1DKE;
1DXT;
1DXU;
1DXV;
1FN3;
1G9V;
1GBU;
1GBV;
1GLI;
1GZX;
1HAB;
1HAC;
1HBA;
1HBB;
1HBS;
1HCO;
1HDB;
1HGA;
1HGB;
1HGC;
1HHO;
1IRD;
1J3Y;
1J3Z;
1J40;
1J41;
1J7S;
1J7W;
1J7Y;
1JY7;
1K0Y;
1K1K;
1KD2;
1LFL;
1LFQ;
1LFT;
1LFV;
1LFY;
1LFZ;
1LJW;
1M9P;
1MKO;
1NEJ;
1NIH;
1NQP;
1O1I;
1O1J;
1O1K;
1O1L;
1O1M;
1O1N;
1O1O;
1O1P;
1QI8;
1QSH;
1QSI;
1QXD;
1QXE;
1R1X;
1R1Y;
1RPS;
1RQ3;
1RQ4;
1RQA;
1RVW;
1SDK;
1SDL;
1THB;
1UIW;
1VWT;
1XXT;
1XY0;
1XYE;
1XZ2;
1XZ4;
1XZ5;
1XZ7;
1XZU;
1XZV;
1Y09;
1Y0A;
1Y0C;
1Y0D;
1Y0T;
1Y0W;
1Y22;
1Y2Z;
1Y31;
1Y35;
1Y45;
1Y46;
1Y4B;
1Y4F;
1Y4G;
1Y4P;
1Y4Q;
1Y4R;
1Y4V;
1Y5F;
1Y5J;
1Y5K;
1Y7C;
1Y7D;
1Y7G;
1Y7Z;
1Y83;
1Y85;
1Y8W;
1YDZ;
1YE0;
1YE1;
1YE2;
1YEN;
1YEO;
1YEQ;
1YEU;
1YEV;
1YFF;
1YG5;
1YGD;
1YGF;
1YH9;
1YHE;
1YHR;
1YIE;
1YIH;
1YVQ;
1YVT;
1YZI;
2D5Z;
2D60;
2DN1;
2DN2;
2DN3;
2DXM;
2H35;
2HBC;
2HBD;
2HBE;
2HBF;
2HBS;
2HCO;
2HHB;
2HHD;
2HHE;
2M6Z;
2W6V;
2W72;
2YRS;
3B75;
3D17;
3D7O;
3DUT;
3HHB;
3HXN;
3IC0;
3IC2;
3KMF;
3NL7;
3NMM;
3ODQ;
3ONZ;
3OO4;
3OO5;
3P5Q;
3QJB;
3QJC;
3QJD;
3QJE;
3R5I;
3S65;
3S66;
3SZK;
3W4U;
3WCP;
3WHM;
4FC3;
4HHB;
4IJ2;
4L7Y;
4M4A;
4M4B;
4MQC;
4MQG;
4MQH;
4MQI;
4N7N;
4N7O;
4N7P;
4N8T;
4NI0;
4NI1;
4ROL;
4ROM;
4WJG;
4X0L;
4XS0;
5E29;
5E6E;
5E83;
5EE4;
5HU6;
5HY8;
5JDO;
5KDQ;
5KSI;
5KSJ;
5NI1;
5SW7;
5U3I;
5UCU;
5UFJ;
5URC;
5VMM;
5WOG;
5WOH;
5X2R;
5X2S;
5X2T;
5X2U;
6BB5;
6BNR;
6BWP;
6BWU;
6DI4;
6FQF;
6HAL;
6HBW;
6HK2;
6KA9;
6KAE;
6KAH;
6KAI;
6KAO;
6KAP;
6KAQ;
6KAR;
6KAS;
6KAT;
6KAU;
6KAV;
6KYE;
6L5V;
6L5W;
6L5X;
6L5Y;
6LCW;
6LCX;
6NBC;
6NBD;
6NQ5;
6TB2;
6XD9;
6XDT;
6XE7;
7AET;
7AEU;
7AEV;
7CUE;
7DY3;
7DY4;
7JJQ;
7JXZ;
7JY0;
7JY1;
7JY3;
7VDE;
|
Mapped Pubmed ID |
10080899;
10390349;
10512707;
10529219;
10611283;
10698631;
10791557;
10794410;
10856700;
10930827;
10930828;
11043018;
11082038;
11111119;
11132230;
11132233;
11159543;
11186256;
11196644;
11300346;
11316875;
11480785;
11570721;
11582643;
11724557;
11741197;
11778661;
11782463;
11800015;
11804203;
11804364;
11812133;
11822023;
11823440;
11827978;
11833853;
11843288;
11843884;
11857746;
11860449;
11869935;
11877258;
11890201;
11895428;
11909945;
11914488;
11939510;
11939511;
11939513;
11965279;
11971871;
11997516;
11999355;
12034837;
12054662;
12077435;
12082507;
12217699;
12217813;
12242335;
12360531;
12394345;
12419253;
12454461;
1252417;
12555809;
12581187;
12581188;
12629213;
12659864;
12709368;
12709369;
12736816;
12773618;
12799453;
12846902;
12865230;
12920025;
12941700;
12955718;
13342742;
13472068;
1390648;
1395;
14529320;
1453464;
14585970;
14587041;
14627823;
14642006;
14645507;
14649314;
14649315;
14649317;
14649318;
14649320;
14705937;
14722738;
14738432;
14958;
15060158;
15073633;
15084587;
1512262;
15131110;
15147217;
15153712;
15174051;
15198986;
15236579;
15256071;
15307413;
15315795;
15327529;
15341482;
15385559;
15448162;
15476181;
15481884;
15481897;
15482674;
15495251;
1552945;
15538648;
15555579;
15565159;
15591902;
15599663;
15607699;
15609277;
15615768;
15658186;
15692663;
15713129;
15727248;
15727901;
15741796;
15751957;
15767753;
15793780;
15795541;
15795925;
15835899;
15858218;
15858266;
15870261;
15882059;
15885239;
15921164;
15938624;
15948210;
15956966;
15973412;
15979088;
16046400;
16085752;
16103715;
16156863;
16156939;
16169070;
16184579;
1618774;
16189277;
16199858;
16227618;
16254903;
16356170;
16403522;
16418531;
16466947;
16470532;
16480967;
16508016;
16537808;
16637741;
16722361;
16765986;
16778136;
16798637;
16848606;
16855288;
16858401;
16859949;
16877501;
16885058;
16898315;
16943425;
16956833;
16990440;
16992238;
16994616;
17008602;
17056066;
17060917;
17096035;
17102136;
17133428;
17135308;
17140342;
17158930;
17160997;
17196618;
17301974;
17339197;
17394396;
17445142;
17446270;
17486493;
17486497;
17490901;
17508724;
17516066;
17519411;
17564253;
17565724;
17612748;
17637845;
17654071;
17654072;
17654503;
17665502;
17720709;
17724704;
17767159;
17845074;
17881306;
17894837;
17916326;
17918249;
17920577;
17925082;
17961316;
17990881;
18022136;
18026953;
18056715;
18071703;
18080800;
18081706;
18096416;
18235968;
18245381;
18257074;
18266765;
18291005;
18294253;
18308336;
18339318;
18381244;
18391696;
18392554;
18392622;
18414926;
18419131;
18428016;
18443145;
18458302;
18461170;
18473240;
18473247;
18473248;
18473944;
18498386;
18586828;
18603555;
18630930;
18644871;
18654889;
18667698;
18694524;
18728021;
18752444;
18759082;
18772456;
18928603;
18932071;
18951049;
1896430;
19065337;
19066892;
19122960;
19127788;
19149873;
19205970;
19205975;
19205976;
19205978;
19215610;
19230568;
19245215;
19247486;
19254853;
19269866;
19288145;
19290524;
19317913;
19320245;
19324878;
19333781;
19346882;
19359777;
19365817;
19372376;
19373584;
19397368;
19465909;
19474452;
19479992;
19488752;
19503809;
19533614;
19556751;
19609526;
19615732;
19618018;
19657833;
19775947;
19843387;
19903589;
19913121;
19954365;
19958184;
19958185;
19958186;
19958187;
19958196;
19958198;
19958201;
19968714;
20036801;
20068219;
20080971;
20113286;
20113295;
20113296;
20162361;
20181291;
20193381;
20230396;
20230836;
20231425;
20305663;
20336466;
20353347;
20353350;
20353351;
20353353;
20353593;
20395516;
20406103;
20430887;
20524821;
20552021;
20557680;
20598923;
20628086;
20628988;
20642332;
20676097;
20711500;
20739079;
20797626;
20798489;
20830473;
20938172;
20943106;
21034280;
21041929;
21068433;
21075280;
21075281;
21075288;
21077758;
21077760;
21086180;
21123872;
21129264;
21173165;
21193395;
21194254;
21194265;
21219849;
21232998;
21238931;
21250885;
21282471;
21333566;
21378272;
21389146;
21523319;
21636902;
21703224;
21757944;
21797700;
21797703;
21801233;
21816820;
21828133;
21863786;
21881051;
21917915;
21931690;
21947948;
21978377;
22028795;
22036762;
2204156;
22075726;
22117603;
22164220;
22178075;
22183073;
22216176;
22217259;
22260316;
22273838;
22289203;
22323332;
22324317;
22427940;
22524255;
22563936;
22625666;
22654577;
22672614;
22675570;
22705794;
22734501;
22734587;
22738610;
22862814;
22898041;
22922649;
22943051;
22981786;
23085440;
23089702;
23101664;
23215760;
23235792;
23272095;
23281611;
23349856;
23361909;
23398055;
23437895;
23457306;
23470150;
23500448;
23577918;
23586372;
23600595;
23638175;
23650620;
23671278;
23683733;
2370669;
23826747;
2383372;
23889802;
23901897;
23915319;
23925429;
23952145;
24074341;
24074398;
24080366;
24080465;
24086375;
24120603;
24149619;
24182909;
24401016;
24425866;
24471793;
24471820;
24578002;
24599423;
24635037;
24769418;
24795058;
24802353;
24857170;
24878022;
25061917;
2506737;
25123009;
25139985;
25155404;
25155936;
25170120;
25183384;
25188417;
25222042;
25222044;
25238043;
25268796;
25271992;
25287606;
25347256;
25370867;
25405919;
25410714;
25412720;
25469539;
25476778;
25497229;
25523871;
25572182;
25572184;
25572186;
25572187;
25617386;
25681747;
25712976;
25849334;
25856402;
25898735;
25918715;
25961502;
25974708;
25976460;
26042536;
26057669;
26097845;
26182728;
26214904;
26292035;
26372288;
26375765;
26445879;
26524961;
26554738;
26582149;
26671256;
26754300;
26840243;
26866725;
26890443;
26928674;
26949259;
26995402;
26997009;
27026582;
27083048;
27085422;
27105433;
27301940;
27377386;
27378309;
2738915;
27529207;
27739611;
27793844;
27828729;
2799391;
28003571;
28181495;
28199095;
28213526;
28224676;
28337324;
28366026;
28374749;
28385923;
28399542;
28465180;
28475449;
28595636;
28665412;
28772282;
28800727;
28858508;
28883619;
28931607;
29064674;
29109153;
29127281;
29127676;
29157167;
29240028;
29246762;
29626215;
29634905;
29655608;
29710269;
29857171;
29964138;
30183107;
30289405;
30289410;
30309760;
30622282;
30833564;
30844732;
31084366;
31146650;
31167908;
3119859;
31246535;
31275994;
31304856;
3136125;
31509281;
31636731;
31793705;
31840728;
31869403;
31899996;
31903828;
31914830;
31939318;
32001505;
32071219;
32078010;
32079421;
32091272;
32108581;
32172616;
32219817;
32370567;
32433728;
32527859;
32638316;
32664780;
32674615;
32679799;
32755585;
33049600;
33066815;
33198537;
33205981;
33219275;
33356244;
33440169;
33461216;
33476375;
33556202;
33723266;
33726930;
33734896;
33798832;
33843396;
33851260;
3427020;
34271589;
34311670;
34389729;
34585664;
34697800;
34907237;
35023007;
35052472;
3938604;
4008491;
4203704;
4327491;
4514311;
4647257;
4975618;
5135507;
5656372;
6094553;
6417382;
6644819;
6683087;
7263686;
7378053;
7541176;
7723849;
7929044;
8114096;
8114097;
8300612;
8415693;
8448109;
8639491;
8639677;
8647854;
8662539;
8855948;
9032082;
9038169;
9151685;
9223274;
9325099;
9441940;
9649306;
9761903;
9876125;
9894000;
|
Motif |
|
Gene Encoded By |
|
Mass |
15,998 |
Kinetics |
|
Metal Binding |
METAL 64; /note=Iron (heme b distal ligand); METAL 93; /note=Iron (heme b proximal ligand) |
Rhea ID |
|
Cross Reference Brenda |
|