Detail Information for IndEnz0018001220
IED ID IndEnz0018001220
Enzyme Type ID peroxidase001220
Protein Name Glutathione S-transferase D1
EC 2.5.1.18
EC 4.5.1.1
DDT-dehydrochlorinase
Gene Name GstD1 GST Gst1 GSTD1-1 CG10045
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MVDFYYLPGSSPCRSVIMTAKAVGVELNKKLLNLQAGEHLKPEFLKINPQHTIPTLVDNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFKKIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAKFEISKYANVNRWYENAKKVTPGWEENWAGCLEFKKYFE
Enzyme Length 209
Uniprot Accession Number P20432
Absorption
Active Site
Activity Regulation
Binding Site BINDING 10; /note=Glutathione; /evidence=ECO:0000269|PubMed:20417639
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:20417639, ECO:0000269|PubMed:22082028}; CATALYTIC ACTIVITY: Reaction=1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride + H(+); Xref=Rhea:RHEA:19217, ChEBI:CHEBI:15378, ChEBI:CHEBI:16130, ChEBI:CHEBI:16598, ChEBI:CHEBI:17996; EC=4.5.1.1; Evidence={ECO:0000269|PubMed:20417639};
DNA Binding
EC Number 2.5.1.18; 4.5.1.1
Enzyme Function FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028, PubMed:20417639). Has DDT dehydrochlorinase activity (PubMed:20417639). May be involved in detoxification (PubMed:22082028). {ECO:0000269|PubMed:20417639, ECO:0000269|PubMed:22082028}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (6); Binding site (1); Chain (1); Domain (2); Erroneous initiation (1); Helix (13); Region (2); Turn (1)
Keywords 3D-structure;Detoxification;Direct protein sequencing;Lyase;Reference proteome;Transferase
Interact With Q9VGA1; Q9VG92
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 3EIN; 3MAK;
Mapped Pubmed ID 10646799; 10773456; 11095759; 11139510; 11157786; 11259658; 11435138; 11583575; 116125; 11792861; 12118001; 12417656; 12443531; 12547198; 12761875; 1445191; 14595091; 14602080; 14605208; 14680800; 14707262; 15094375; 15096208; 15166145; 15464587; 15710747; 17054780; 1731792; 17907271; 17924685; 18039872; 18077584; 18194654; 18245335; 18628398; 18651915; 19237508; 19342413; 19409416; 19627268; 19649249; 19917591; 20080168; 20184862; 20609461; 21036383; 21108635; 21149573; 21228205; 21295275; 21372148; 21406223; 21423626; 21799256; 21987827; 22065747; 22430022; 22475449; 22940110; 23071443; 23211361; 23226288; 23408904; 23438069; 23521918; 23738891; 23792147; 23944235; 23950728; 24092876; 24141879; 24486154; 24621103; 24974374; 25063457; 25193493; 25281658; 25294943; 25310450; 25426014; 25474322; 25931115; 26158631; 26290570; 26344147; 26526100; 26599788; 26694630; 26715182; 26801178; 26861501; 26911346; 27080728; 27374982; 27514026; 27582081; 27585295; 27701059; 27794539; 28024300; 28184905; 28253732; 28344128; 28434858; 28568827; 28634323; 28660301; 28669604; 29091025; 29245136; 29259189; 29357349; 29375370; 29677000; 30110626; 30263675; 30322073; 30478906; 30658986; 30682390; 30739017; 30770378; 30837466; 3085676; 30974046; 31018943; 3104776; 31425511; 31568497; 31636947; 31841522; 32024668; 32284136; 32291952; 32344153; 32424050; 32598968; 32611818; 32663363; 32773682; 32801000; 33109529; 33264304; 33306674; 33425686; 33474790; 33495633; 33547411; 33563832; 33748138; 33777703; 33909998; 33992327; 34204287; 34216545; 34370733; 34568344; 34605405; 34662537; 34737239; 6439209; 7713409; 7743390; 7775439; 7961718; 8384578; 8580911; 8743688; 8896375; 8913769; 9275195; 9321417; 9342399; 9415093; 9514730; 9533124; 9536439; 9927183;
Motif
Gene Encoded By
Mass 23,866
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.23 mM for glutathione {ECO:0000269|PubMed:22082028}; KM=0.45 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:22082028}; Vmax=84.2 umol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:22082028}; Vmax=7.51 umol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate {ECO:0000269|PubMed:22082028}; Vmax=77 nmol/min/mg enzyme with adrenochrome as substrate {ECO:0000269|PubMed:22082028}; Vmax=0.33 umol/min/mg enzyme with phenethyl isothiocyanate as substrate {ECO:0000269|PubMed:22082028}; Vmax=0.517 umol/min/mg enzyme with prostaglandin A2 as substrate {ECO:0000269|PubMed:22082028}; Vmax=0.012 umol/min/mg enzyme with 5-hydroperoxyeicosatetraenoic acid as substrate {ECO:0000269|PubMed:22082028}; Vmax=0.22 umol/min/mg enzyme with 2-hydroxyethyl disulfide as substrate {ECO:0000269|PubMed:22082028};
Metal Binding
Rhea ID RHEA:16437; RHEA:19217
Cross Reference Brenda