Detail Information for IndEnz0018001251
IED ID IndEnz0018001251
Enzyme Type ID peroxidase001251
Protein Name Purple acid phosphatase 17
EC 3.1.3.2
Acid phosphatase type 5
Peroxidase
EC 1.11.1.7
Gene Name PAP17 ACP5 At3g17790 MEB5.10
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MNSGRRSLMSATASLSLLLCIFTTFVVVSNGELQRFIEPAKSDGSVSFIVIGDWGRRGSFNQSLVAYQMGKIGEKIDLDFVVSTGDNFYDNGLFSEHDPNFEQSFSNIYTAPSLQKQWYSVLGNHDYRGDAEAQLSSVLREIDSRWICLRSFVVDAELVEMFFVDTTPFVKEYYTEADGHSYDWRAVPSRNSYVKALLRDLEVSLKSSKARWKIVVGHHAMRSIGHHGDTKELNEELLPILKENGVDLYMNGHDHCLQHMSDEDSPIQFLTSGAGSKAWRGDINPVTINPKLLKFYYDGQGFMSARFTHSDAEIVFYDVFGEILHKWVTSKQLLHSSV
Enzyme Length 338
Uniprot Accession Number Q9SCX8
Absorption
Active Site ACT_SITE 227; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation ACTIVITY REGULATION: Inhibited by phosphate and molybdate. {ECO:0000269|PubMed:10504579}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2; CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
DNA Binding
EC Number 3.1.3.2; 1.11.1.7
Enzyme Function FUNCTION: Metallo-phosphoesterase involved in phosphate metabolism. Has a peroxidase activity. {ECO:0000269|PubMed:10504579}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Glycosylation (1); Metal binding (8); Region (1); Sequence conflict (3); Signal peptide (1)
Keywords Glycoprotein;Hydrolase;Iron;Metal-binding;Oxidoreductase;Reference proteome;Secreted;Signal;Zinc
Interact With
Induction INDUCTION: By phosphate starvation, during senescence, by ABA, by H(2)O(2), and by salt stress. {ECO:0000269|PubMed:10504579}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..31; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12432076; 14617064; 15681455; 16244138; 16673935; 16679424; 16963519; 17085506; 17085508; 17346263; 17408486; 17419847; 17559508; 18296723; 18506479; 18650403; 18684332; 18775970; 21546457; 25697796; 27181950; 28627464; 33101335;
Motif
Gene Encoded By
Mass 38,297
Kinetics
Metal Binding METAL 53; /note=Iron; /evidence=ECO:0000250; METAL 86; /note=Iron; /evidence=ECO:0000250; METAL 86; /note=Zinc; /evidence=ECO:0000250; METAL 89; /note=Iron; /evidence=ECO:0000250; METAL 124; /note=Zinc; /evidence=ECO:0000250; METAL 218; /note=Zinc; /evidence=ECO:0000250; METAL 253; /note=Zinc; /evidence=ECO:0000250; METAL 255; /note=Iron; /evidence=ECO:0000250
Rhea ID RHEA:15017; RHEA:56136
Cross Reference Brenda