| IED ID | IndEnz0018001256 |
| Enzyme Type ID | peroxidase001256 |
| Protein Name |
Linoleate diol synthase EC 1.13.11.60 EC 5.4.4.6 Linoleate 8R -dioxygenase Linoleate 8-dioxygenase |
| Gene Name | |
| Organism | Gaeumannomyces graminis var. graminis (Turf grass take-all root rot fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Magnaporthaceae Gaeumannomyces Gaeumannomyces graminis var. graminis (Turf grass take-all root rot fungus) |
| Enzyme Sequence | MTVSTHHDDSPGLSGRLRDLLHHVFGNQKSPTVYPNAPGNSAKPVPTGLADDIDKLGFKDIDTLLIFLNSAVKGVNDDQQFLLEKMIQLLAKLPPASREGKKLTDGLINDLWDSLDHPPVASLGKGFSFREPDGSNNNIHLPSLGAANTPYARSTKPLVFQNPNPPDPATIFDTLMVRDPAKFRPHPNKISSMLFYLATIITHDIFQTSPRDFNINLTSSYLDLSPLYGRNHDEQMAVRTGKDGLLKPDTFSSKRVIGFPPGVGAFLIMFNRFHNYVVTQLAKINEGGRFKRPTTPDDTAGWETYDNSLFQTGRLITCGLYINIVLGDYVRTILNLNRANTTWNLDPRTKEGKSLLSKPTPEAVGNQVSVEFNLIYRWHCTISERDDKWTTNAMREALGGQDPATAKMEDVMRALGMFEKNIPDEPEKRTLAGLTRQSDGAFDDTELVKILQESIEDVAGAFGPNHVPACMRAIEILGIKQSRTWNVATLNEFRQFIGLTPHDSFYHMNPDPKICKILAQMYDSPDAVELYPGIMAEAAKPPFSPGSGLCPPYTTSRAILSDAVSLVRGDRFYTVDYTPRNITNWGFNEASTDKAVDWGHVIYKLFFRAFPNHFLPNSVYAHFPFVVPSENKLIFEGLGAANKYSWDPPKARAPIQFIRSHKAVLEVLSNQKDYKVTWGPAIKMLSGDPATSFALAGDEPANAASRHHVIAALTAPKQWRDEVRRFYEVTTRDLLRRHGAPVHGVGAGPRTHEVDVIRDVIGLAHARFMASLFSLPLKEEGKEEGAYGEHELYRSLVTIFAAIFWDSDVCNSLKLHQASKAAADKMSALIAEHVREMEAGTGFLGALGKLKDLITGNDVHANGNGVYTNGNGVYTNGNGVHTNGNGVHTNGNGVPHAAPSLRSFGDQLLQRMLSQDGRSIEETVSGTILPVVMAGTANQTQLLAQCLDYYLGVGEKHLPEMKRLAMLNTSEADEKLLKYTMEGCRIRGCVALYRAVVTDQAVDDTIPCIPNKDDPTFARPLSNPQVAESARTLKLSTGTRMLVDLTTASHDPAAFPDPDEVRLDRPLESYVHFGLGPHRCAGEPISQIALSSVMKVLLQLDGLRRAAGPRGEIRSYPASQWPGQAGRPPRDPAWSGLRTFTSADQSAFSPLATTMKINWEGRGDL |
| Enzyme Length | 1165 |
| Uniprot Accession Number | Q9UUS2 |
| Absorption | |
| Active Site | ACT_SITE 376; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienoate + O2 = (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate; Xref=Rhea:RHEA:25395, ChEBI:CHEBI:15379, ChEBI:CHEBI:30245, ChEBI:CHEBI:58659; EC=1.13.11.60; Evidence={ECO:0000269|PubMed:8662736}; CATALYTIC ACTIVITY: Reaction=(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate = (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate; Xref=Rhea:RHEA:25399, ChEBI:CHEBI:57468, ChEBI:CHEBI:58659; EC=5.4.4.6; Evidence={ECO:0000269|PubMed:8662736}; |
| DNA Binding | |
| EC Number | 1.13.11.60; 5.4.4.6 |
| Enzyme Function | FUNCTION: Catalyzes the dioxygenation of linoleic acid to (8R)-hydroperoxylinoleate and isomerization of the resulting hydroperoxide to (7S,8S)-dihydroxylinoleate. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.2-7.4. {ECO:0000269|PubMed:8662736}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (2); Region (1) |
| Keywords | Dioxygenase;Direct protein sequencing;Heme;Iron;Isomerase;Metal-binding;Oxidoreductase;Peroxidase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 128,322 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 uM for oxygen {ECO:0000269|PubMed:8662736}; KM=8 uM for linoleic acid {ECO:0000269|PubMed:8662736}; Vmax=2.2 umol/min/mg enzyme toward oxygen {ECO:0000269|PubMed:8662736}; Vmax=4 umol/min/mg enzyme toward linoleic acid {ECO:0000269|PubMed:8662736}; |
| Metal Binding | METAL 203; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; METAL 379; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00298 |
| Rhea ID | RHEA:25395; RHEA:25399 |
| Cross Reference Brenda | 1.13.11.60;5.4.4.6; |