| IED ID | IndEnz0018001409 |
| Enzyme Type ID | peroxidase001409 |
| Protein Name |
E3 ubiquitin-protein ligase SlrP EC 2.3.2.27 RING-type E3 ubiquitin transferase SlrP Secreted effector protein SlrP |
| Gene Name | slrP STM14_928 |
| Organism | Salmonella typhimurium (strain 14028s / SGSC 2262) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella typhimurium (strain 14028s / SGSC 2262) |
| Enzyme Sequence | MFNITNIQSTARHQSISNEASTEVPLKEEIWNKISAFFSSEHQVEAQNCIAYLCHPPETASPEEIKSKFECLRMLAFPAYADNIQYSRGGADQYCILSENSQEILSIVFNTEGYTVEGGGKSVTYTRVTESEQASSASGSKDAVNYELIWSEWVKEAPAKEAANREEAVQRMRDCLKNNKTELRLKILGLTTIPAYIPEQITTLILDNNELKSLPENLQGNIKTLYANSNQLTSIPATLPDTIQEMELSINRITELPERLPSALQSLDLFHNKISCLPENLPEELRYLSVYDNSIRTLPAHLPSEITHLNVQSNSLTALPETLPPGLKTLEAGENALTSLPASLPPELQVLDVSKNQITVLPETLPPTITTLDVSRNALTNLPENLPAALQIMQASRNNLVRLPESLPHFRGEGPQPTRIIVEYNPFSERTIQNMQRLMSSVDYQGPRVLFAMGDFSIVRVTRPLHQAVQGWLTSLEEEDVNQWRAFEAEANAAAFSGFLDYLGDTQNTRHPDFKEQVSAWLMRLAEDSALRETVFIIAMNATISCEDRVTLAYHQMQEATLVHDAERGAFDSHLAELIMAGREIFRLEQIESLAREKVKRLFFIDEVEVFLGFQNQLRESLSLTTMTRDMRFYNVSGITESDLDEAEIRIKMAENRDFHKWFALWGPWHKVLERIAPEEWREMMAKRDECIETDEYQSRVNAELEDLRIADDSDAERTTEVQMDAERAIGIKIMEEINQTLFTEIMENILLKKEVSSLMSAYWR |
| Enzyme Length | 765 |
| Uniprot Accession Number | D0ZRB2 |
| Absorption | |
| Active Site | ACT_SITE 546; /note=Glycyl thioester intermediate; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; |
| DNA Binding | |
| EC Number | 2.3.2.27 |
| Enzyme Function | FUNCTION: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Can ubiquitinate both ubiquitin and host TXN (thioredoxin). Leads to significant decrease of thioredoxin activity and increase of host cell death. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (18); Chain (1); Helix (21); Region (3); Repeat (10); Turn (2) |
| Keywords | 3D-structure;Host cytoplasm;Leucine-rich repeat;Repeat;Secreted;Transferase;Ubl conjugation;Ubl conjugation pathway;Virulence |
| Interact With | P10599 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Host cytoplasm. Note=Secreted via type III secretion systems 1 and 2 (SPI-1 and SPI-2 TTSS), and delivered into the host cytoplasm. |
| Modified Residue | |
| Post Translational Modification | PTM: Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and ubiquitin. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4PUF; |
| Mapped Pubmed ID | 19690162; 25184225; |
| Motif | |
| Gene Encoded By | |
| Mass | 86,944 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |