Detail Information for IndEnz0018001410
IED ID IndEnz0018001410
Enzyme Type ID peroxidase001410
Protein Name O-methyltransferase SfmM3
EC 2.1.1.-
Gene Name sfmM3
Organism Streptomyces lavendulae
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces lavendulae
Enzyme Sequence MRRMLYAQLPSRALVVVAQLGIADILAEGPADISTLAERTSTDAVALARLLRGLAVFGVFEEGAEQVYSLTPLGEALTSGHPASALPSATLVAGQFGAAWGDLLETVRTGQSPFERSRGVSLFTHMEQDEELRAVFDDSQGRGLALELDEILRAIDFSAYPTVVDVGGSDGTFLRRILSAHPDISGIVFDLPGSTSLQAERPTADPLEGRYSVATGDFFDSLPEGGDLYLLSHILHDWDDDRAVQILRTCRAAMSDDATLMVVDLIAANRGQRDERLHTAALMDLYMLSLFGGNGGQERTAAQVEVLLSKAGFRITRVDSLPSGMNVIRAVRAA
Enzyme Length 334
Uniprot Accession Number B0CN39
Absorption
Active Site ACT_SITE 236; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01020
Activity Regulation
Binding Site BINDING 190; /note=S-adenosyl-L-methionine; /evidence=ECO:0000255|PROSITE-ProRule:PRU01020
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=5-hydroxy-3-methyl-L-tyrosine + S-adenosyl-L-methionine = 5-hydroxy-3-methyl-O-methyl-L-tyrosine + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:47752, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:78241, ChEBI:CHEBI:87846; Evidence={ECO:0000305|PubMed:22187429};
DNA Binding
EC Number 2.1.1.-
Enzyme Function FUNCTION: O-methyltransferase that mediates the methylation of 3-hydroxy-5-methyl-L-tyrosine (3-OH-5-Me-Tyr) into 3-hydroxy-5-methyl-O-methyltyrosine (3-OH-5-Me-OMe-Tyr), a core structure of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family. {ECO:0000305|PubMed:22187429}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Antibiotic biosynthesis. {ECO:0000269|PubMed:19494690}.
nucleotide Binding
Features Active site (1); Binding site (1); Chain (1); Region (1)
Keywords Antibiotic biosynthesis;Methyltransferase;S-adenosyl-L-methionine;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 35,860
Kinetics
Metal Binding
Rhea ID RHEA:47752
Cross Reference Brenda