| IED ID | IndEnz0018001424 |
| Enzyme Type ID | peroxidase001424 |
| Protein Name |
Dye-decolorizing peroxidase DyP EC 1.11.1.7 |
| Gene Name | dyp Rv0799c |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Enzyme Sequence | MAVPAVSPQPILAPLTPAAIFLVATIGADGEATVHDALSKISGLVRAIGFRDPTKHLSVVVSIGSDAWDRLFAGPRPTELHPFVELTGPRHTAPATPGDLLFHIRAETMDVCFELAGRILKSMGDAVTVVDEVHGFRFFDNRDLLGFVDGTENPSGPIAIKATTIGDEDRNFAGSCYVHVQKYVHDMASWESLSVTEQERVIGRTKLDDIELDDNAKPANSHVALNVITDDDGTERKIVRHNMPFGEVGKGEYGTYFIGYSRTPTVTEQMLRNMFLGDPAGNTDRVLDFSTAVTGGLFFSPTIDFLDHPPPLPQAATPTLAAGSLSIGSLKGSPR |
| Enzyme Length | 335 |
| Uniprot Accession Number | I6Y4U9 |
| Absorption | |
| Active Site | ACT_SITE 149; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q47KB1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7; Evidence={ECO:0000269|PubMed:24855650, ECO:0000269|PubMed:34751132}; |
| DNA Binding | |
| EC Number | 1.11.1.7 |
| Enzyme Function | FUNCTION: Cargo of a type 1 encapsulin nanocompartment in situ; this cargo protects against oxidative stress at low pH. When expressed in the cytoplasm (absence of the encapsulin shell gene) it is almost as protective as the intact nanocompartment; its encapsulation has a modest yet significant effect on protection against oxidative stress at low pH (PubMed:34751132). A heme-dependent peroxidase, it probably does not have deferrochelatase activity. Converts guaiacol and H2O2 to tetraguaiacol, also acts on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Retains peroxidase activity when encapsulated but has a reduced set of substrates; acts on ABTS but not guaiacol (PubMed:24855650, PubMed:34751132). {ECO:0000269|PubMed:24855650, ECO:0000269|PubMed:34751132}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.0 or less. {ECO:0000269|PubMed:34751132}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (1); Mutagenesis (1); Region (1) |
| Keywords | Encapsulin nanocompartment;Heme;Iron;Metal-binding;Oxidoreductase;Peroxidase;Reference proteome;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Encapsulin nanocompartment {ECO:0000269|PubMed:24855650}. Note=Consistently identified in encapsulin nanocompartments isolated in situ (PubMed:34751132). Located inside the nanocompartment in E.coli (Probable). {ECO:0000269|PubMed:34751132, ECO:0000305|PubMed:24855650}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 36,048 |
| Kinetics | |
| Metal Binding | METAL 222; /note=Iron (heme proximal ligand); /evidence=ECO:0000250|UniProtKB:Q47KB1 |
| Rhea ID | RHEA:56136 |
| Cross Reference Brenda |