Detail Information for IndEnz0018001430
IED ID IndEnz0018001430
Enzyme Type ID peroxidase001430
Protein Name Manganese peroxidase 2
MnP2
EC 1.11.1.13
Manganese peroxidase isozyme 2
Gene Name mnp2
Organism Phlebia radiata (White-rot fungus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetes incertae sedis Polyporales Meruliaceae Phlebia Phlebia radiata (White-rot fungus)
Enzyme Sequence MAFNFAAILAFVSLAAVTSAAPSKTTCSNGVVVPDAVCCDFVPLASALQSEVLMGDCGEDAHELVRLIFHDAIAISQSMGPSAGGGADGSMLIFPTVEPAFFPNLGIADSVNNLIPFLSQFPTISAGDLVQFAGAVAISNCPGAPQLEFLAGRPNATAPAIDGLIPEPQDDVTKILARFKDAGNFSPAEVVALLASHSIARADHVDPTLDAAPFDSTPFDFDTQIFLEVLLKGVGFPGLANNTGEVSSPLPVTDGTDVGELRLQSDFALARDERTACAWQSFVNEQEAMATAFKNAVKKLAVLGHNRNDLVDCSAVVPVPKPATGTPATFPASTGPQDLELTCTTEPFPTLSTAPGAQQTLIPHCSDGTMTCNSVQFDGPATNFGGADDS
Enzyme Length 390
Uniprot Accession Number Q70LM3
Absorption
Active Site ACT_SITE 70; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00297, ECO:0000255|PROSITE-ProRule:PRU10012"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=2 H(+) + H2O2 + 2 Mn(2+) = 2 H2O + 2 Mn(3+); Xref=Rhea:RHEA:22776, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:29035, ChEBI:CHEBI:29041; EC=1.11.1.13;
DNA Binding
EC Number 1.11.1.13
Enzyme Function FUNCTION: Catalyzes the oxidation of Mn(2+) to Mn(3+). The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds. {ECO:0000269|Ref.2}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (5); Glycosylation (2); Metal binding (12); Sequence conflict (2); Signal peptide (1); Site (1)
Keywords Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Hydrogen peroxide;Iron;Lignin degradation;Manganese;Metal-binding;Oxidoreductase;Peroxidase;Secreted;Signal
Interact With
Induction INDUCTION: By a combination of high manganese and malonate levels. {ECO:0000269|Ref.2}.
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000269|PubMed:15809005
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 40,326
Kinetics
Metal Binding METAL 59; /note=Manganese; /evidence=ECO:0000250; METAL 63; /note=Manganese; /evidence=ECO:0000250; METAL 71; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 86; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 88; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 90; /note=Calcium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 197; /note=Iron (heme b axial ligand); /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 198; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 203; /note=Manganese; /evidence=ECO:0000250; METAL 215; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 217; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297; METAL 222; /note=Calcium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00297
Rhea ID RHEA:22776
Cross Reference Brenda