| IED ID | IndEnz0018001433 |
| Enzyme Type ID | peroxidase001433 |
| Protein Name |
L-tyrosine C 3 -methyltransferase EC 2.1.1.304 |
| Gene Name | sfmM2 |
| Organism | Streptomyces lavendulae |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces lavendulae |
| Enzyme Sequence | MTISLENTTVGQNPAGGPPTGKAPLDMEGLAWILFGASAFQYLNAACELNLFELLENKPGLTKPQIGAELGLADRANDILLLGATATGMLTVEDGRYQLATVLAELLKTDDWQRFKDTVGFEQYVCYEGQIDFTESLRSNSNVGLRRVRGSGRDLYHRLHENPQMEQAFYKYMRSWSELANQHLVEVLDLSGTSKLLDCGGGDAVNSIALAQANPHIEAGILEIPPTAPLTEKKIAEAGLSDRITVKPGDMHTDEFPTGYDTVMFAHQLVIWTPEENTALLRKAYNALPEGGRVIIFNSMSNDEGDGPVVAALDSVYFAALPAEGGMIYSWATYEESLTKAGFNPETFQRIDFPGWTPHGVIIATK |
| Enzyme Length | 366 |
| Uniprot Accession Number | B0CN31 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 223; /note=S-adenosyl-L-methionine; /evidence=ECO:0000255|PROSITE-ProRule:PRU01020 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-tyrosine + S-adenosyl-L-methionine = 3-methyl-L-tyrosine + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:41428, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:58315, ChEBI:CHEBI:59789, ChEBI:CHEBI:78239; EC=2.1.1.304; Evidence={ECO:0000303|PubMed:22187429}; |
| DNA Binding | |
| EC Number | 2.1.1.304 |
| Enzyme Function | FUNCTION: C-methyltransferase that mediates the methylation of tyrosine into 3-methyl-L-tyrosine (3-Me-Tyr) in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family. Involved in biosynthesis of 3-hydroxy-5-methyl-O-methyltyrosine (3-OH-5-Me-OMe-Tyr), a core structure of saframycin A. {ECO:0000269|PubMed:19494690, ECO:0000269|PubMed:22187429}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Antibiotic biosynthesis. {ECO:0000269|PubMed:19494690, ECO:0000269|PubMed:22187429}. |
| nucleotide Binding | |
| Features | Binding site (1); Chain (1); Compositional bias (1); Region (1) |
| Keywords | Antibiotic biosynthesis;Methyltransferase;S-adenosyl-L-methionine;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,990 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:41428 |
| Cross Reference Brenda | 2.1.1.304; |