Detail Information for IndEnz0018001437
IED ID IndEnz0018001437
Enzyme Type ID peroxidase001437
Protein Name Non-secretory ribonuclease
EC 4.6.1.18
Eosinophil cationic-type ribonuclease 4
MR-4
Ribonuclease 2
RNase 2
Gene Name Rnase2 Ear4
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MGPKLLESRLCLLLLLGLVLMLASCQAQILSQKFYTQHIYNSTYPRCDAVMRVVNRYRPRCKDINTFLHTSFADVVAVCGHPNITCNNLTRKNCHASSFQVFITFCNLTMPTRICTQCRYQTTGSVKYYRVACENRTPQDTPMYPVVPVHLDGTF
Enzyme Length 155
Uniprot Accession Number O35291
Absorption
Active Site ACT_SITE 38; /note=Proton acceptor; /evidence=ECO:0000250; ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 33; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784}; CATALYTIC ACTIVITY: Reaction=An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784};
DNA Binding
EC Number 4.6.1.18
Enzyme Function FUNCTION: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Binding site (1); Chain (1); Disulfide bond (4); Glycosylation (4); Modified residue (1); Region (1); Signal peptide (1)
Keywords Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Lyase;Lysosome;Nitration;Nuclease;Reference proteome;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Lysosome {ECO:0000305}. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule.
Modified Residue MOD_RES 57; /note=3'-nitrotyrosine; /evidence=ECO:0000250|UniProtKB:P10153
Post Translational Modification
Signal Peptide SIGNAL 1..25; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 15058383; 15676279;
Motif
Gene Encoded By
Mass 17,723
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda