| IED ID | IndEnz0018001437 |
| Enzyme Type ID | peroxidase001437 |
| Protein Name |
Non-secretory ribonuclease EC 4.6.1.18 Eosinophil cationic-type ribonuclease 4 MR-4 Ribonuclease 2 RNase 2 |
| Gene Name | Rnase2 Ear4 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MGPKLLESRLCLLLLLGLVLMLASCQAQILSQKFYTQHIYNSTYPRCDAVMRVVNRYRPRCKDINTFLHTSFADVVAVCGHPNITCNNLTRKNCHASSFQVFITFCNLTMPTRICTQCRYQTTGSVKYYRVACENRTPQDTPMYPVVPVHLDGTF |
| Enzyme Length | 155 |
| Uniprot Accession Number | O35291 |
| Absorption | |
| Active Site | ACT_SITE 38; /note=Proton acceptor; /evidence=ECO:0000250; ACT_SITE 150; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 33; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784}; CATALYTIC ACTIVITY: Reaction=An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784}; |
| DNA Binding | |
| EC Number | 4.6.1.18 |
| Enzyme Function | FUNCTION: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Binding site (1); Chain (1); Disulfide bond (4); Glycosylation (4); Modified residue (1); Region (1); Signal peptide (1) |
| Keywords | Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Lyase;Lysosome;Nitration;Nuclease;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000305}. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule. |
| Modified Residue | MOD_RES 57; /note=3'-nitrotyrosine; /evidence=ECO:0000250|UniProtKB:P10153 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15058383; 15676279; |
| Motif | |
| Gene Encoded By | |
| Mass | 17,723 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |