| IED ID | IndEnz0018001446 |
| Enzyme Type ID | peroxidase001446 |
| Protein Name |
Non-secretory ribonuclease EC 4.6.1.18 Eosinophil-derived neurotoxin RNase UpI-2 Ribonuclease 2 RNase 2 Ribonuclease US |
| Gene Name | RNASE2 EDN RNS2 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MVPKLFTSQICLLLLLGLLAVEGSLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTFLLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDNRDQRRDPPQYPVVPVHLDRII |
| Enzyme Length | 161 |
| Uniprot Accession Number | P10153 |
| Absorption | |
| Active Site | ACT_SITE 42; /note=Proton acceptor; ACT_SITE 156; /note=Proton donor |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784}; CATALYTIC ACTIVITY: Reaction=An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).; EC=4.6.1.18; Evidence={ECO:0000250|UniProtKB:P47784}; |
| DNA Binding | |
| EC Number | 4.6.1.18 |
| Enzyme Function | FUNCTION: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. {ECO:0000269|PubMed:12578357, ECO:0000269|PubMed:3458170}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (6); Chain (1); Disulfide bond (4); Glycosylation (6); Helix (4); Modified residue (1); Natural variant (2); Region (1); Sequence conflict (4); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Chemotaxis;Direct protein sequencing;Disulfide bond;Endonuclease;Glycoprotein;Hydrolase;Lyase;Lysosome;Nitration;Nuclease;Reference proteome;Signal |
| Interact With | Q15700 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000305}. Cytoplasmic granule. Note=Matrix of eosinophil's large specific granule. |
| Modified Residue | MOD_RES 60; /note=3'-nitrotyrosine; /evidence=ECO:0000269|PubMed:18694936 |
| Post Translational Modification | PTM: A particular signal processing and glycosylation pattern may differentiate the UpI2 RNase, found specifically in pregnant women urine, from other nonsecretory RNases. {ECO:0000269|PubMed:7947762, ECO:0000269|PubMed:9450956}. |
| Signal Peptide | SIGNAL 1..27; /evidence="ECO:0000269|PubMed:3166997, ECO:0000269|PubMed:3182786, ECO:0000269|PubMed:3458170, ECO:0000269|PubMed:3926759, ECO:0000269|PubMed:8471426" |
| Structure 3D | X-ray crystallography (13) |
| Cross Reference PDB | 1GQV; 1HI2; 1HI3; 1HI4; 1HI5; 1K2A; 2BEX; 2BZZ; 2C01; 2C02; 2C05; 5E13; 6SSO; |
| Mapped Pubmed ID | 11876642; 11916383; 11959927; 12606041; 15356558; 15528350; 16014847; 16428483; 17351112; 17641730; 17927842; 17965582; 18195069; 18636464; 19014520; 19115260; 19717523; 21680952; 23053729; 23533305; 24814827; 25221852; 26534742; 26551065; 28169973; 28866306; 30367910; 32294405; 32571644; 8692836; 9826755; |
| Motif | |
| Gene Encoded By | |
| Mass | 18,354 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |