Detail Information for IndEnz0018001577
IED ID IndEnz0018001577
Enzyme Type ID peroxidase001577
Protein Name Putative peroxiredoxin ycf42
EC 1.11.1.24
Thioredoxin peroxidase
Gene Name ycf42
Organism Porphyra purpurea (Red seaweed) (Ulva purpurea)
Taxonomic Lineage cellular organisms Eukaryota Rhodophyta Bangiophyceae Bangiales Bangiaceae Porphyra Porphyra purpurea (Red seaweed) (Ulva purpurea)
Enzyme Sequence MISGHNCLQVGQIAPDFSATAVYDQEFKTIKLSDFKNKYVILFFYPLDFTFVCPTEITAFSDKYSDFSELNTEILGVSVDSEYSHLAWLQTDRESGGLGDLEYPLVSDLKKEISIAYNVLNSGGVALRGLFIIDPKGIIQYSTVNNLEFGRSVEETLRVLQAIQYVQAHPDEVCPANWKPGDRTMNPDPIKSKNYFAAA
Enzyme Length 199
Uniprot Accession Number P51272
Absorption
Active Site ACT_SITE 53; /note=Cysteine sulfenic acid (-SOH) intermediate; /evidence=ECO:0000250|UniProtKB:Q06830
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=1.11.1.24; Evidence={ECO:0000250|UniProtKB:Q06830};
DNA Binding
EC Number 1.11.1.24
Enzyme Function FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. {ECO:0000250|UniProtKB:Q06830}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (2); Domain (1)
Keywords Antioxidant;Chloroplast;Disulfide bond;Oxidoreductase;Peroxidase;Plastid;Redox-active center
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: The Cys-53-SH group is the primary site of oxidation by H(2)O(2), and the oxidized Cys-53 (probably Cys-SOH) rapidly reacts with Cys-174-SH of the other subunit to form an intermolecular disulfide. This disulfide is subsequently reduced by thioredoxin (By similarity). {ECO:0000250}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By Chloroplast
Mass 22,282
Kinetics
Metal Binding
Rhea ID RHEA:62620
Cross Reference Brenda