| IED ID | IndEnz0018001594 |
| Enzyme Type ID | peroxidase001594 |
| Protein Name |
Peroxiredoxin-2F, mitochondrial EC 1.11.1.25 Glutaredoxin-dependent peroxiredoxin Peroxiredoxin IIF Thioredoxin peroxidase 2F |
| Gene Name | PRXIIF At3g06050 F24F17.3 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAMSILKLRNLSALRSAANSARIGVSSRGFSKLAEGTDITSAAPGVSLQKARSWDEGVSSKFSTTPLSDIFKGKKVVIFGLPGAYTGVCSQQHVPSYKSHIDKFKAKGIDSVICVSVNDPFAINGWAEKLGAKDAIEFYGDFDGKFHKSLGLDKDLSAALLGPRSERWSAYVEDGKVKAVNVEEAPSDFKVTGAEVILGQI |
| Enzyme Length | 201 |
| Uniprot Accession Number | Q9M7T0 |
| Absorption | |
| Active Site | ACT_SITE 89; /note=Cysteine sulfenic acid (-SOH) intermediate; /evidence=ECO:0000250|UniProtKB:A9PCL4 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[glutaredoxin]-dithiol + a hydroperoxide = [glutaredoxin]-disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62624, Rhea:RHEA-COMP:10729, Rhea:RHEA-COMP:10730, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924, ChEBI:CHEBI:50058; EC=1.11.1.25; Evidence={ECO:0000269|PubMed:15632145}; |
| DNA Binding | |
| EC Number | 1.11.1.25 |
| Enzyme Function | FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Reduces preferentially hydrogen peroxide rather than alkyl peroxides. May be involved in mitochondrial redox homeostasis. {ECO:0000269|PubMed:15632145}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Erroneous initiation (1); Modified residue (2); Sequence conflict (1); Transit peptide (1) |
| Keywords | Antioxidant;Direct protein sequencing;Mitochondrion;Oxidoreductase;Peroxidase;Phosphoprotein;Redox-active center;Reference proteome;Stress response;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By oxidative stress. {ECO:0000269|PubMed:12492832}. |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:11743114, ECO:0000269|PubMed:12492832, ECO:0000269|PubMed:14671022, ECO:0000269|PubMed:15632145, ECO:0000305|PubMed:25732537}. |
| Modified Residue | MOD_RES 37; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:22092075; MOD_RES 149; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22092075 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11997378; 12529539; 12626114; 15032878; 15998247; 16242667; 16247729; 16299171; 16502469; 16606633; 16915352; 16927206; 17092311; 17376161; 18218973; 18385124; 18493039; 18650403; 18775970; 18805951; 18820081; 20118269; 21146842; 28627464; 28782990; |
| Motif | |
| Gene Encoded By | |
| Mass | 21,445 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:62624 |
| Cross Reference Brenda |