| IED ID | IndEnz0018001643 |
| Enzyme Type ID | peroxidase001643 |
| Protein Name |
Chloroperoxidase EC 1.11.1.10 Chloride peroxidase CPO |
| Gene Name | CPO |
| Organism | Leptoxyphium fumago (Caldariomyces fumago) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta dothideomyceta Dothideomycetes Dothideomycetidae Capnodiales Capnodiaceae Leptoxyphium Leptoxyphium fumago (Caldariomyces fumago) |
| Enzyme Sequence | MFSKVLPFVGAVAALPHSVRQEPGSGIGYPYDNNTLPYVAPGPTDSRAPCPALNALANHGYIPHDGRAISRETLQNAFLNHMGIANSVIELALTNAFVVCEYVTGSDCGDSLVNLTLLAEPHAFEHDHSFSRKDYKQGVANSNDFIDNRNFDAETFQTSLDVVAGKTHFDYADMNEIRLQRESLSNELDFPGWFTESKPIQNVESGFIFALVSDFNLPDNDENPLVRIDWWKYWFTNESFPYHLGWHPPSPAREIEFVTSASSAVLAASVTSTPSSLPSGAIGPGAEAVPLSFASTMTPFLLATNAPYYAQDPTLGPNDKREAAPAATTSMAVFKNPYLEAIGTQDIKNQQAYVSSKAAAMASAMAANKARNL |
| Enzyme Length | 373 |
| Uniprot Accession Number | P04963 |
| Absorption | |
| Active Site | ACT_SITE 204 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O.; EC=1.11.1.10; |
| DNA Binding | |
| EC Number | 1.11.1.10 |
| Enzyme Function | FUNCTION: Catalyzes peroxidative halogenations involved in the biosynthesis of clardariomycin (2,2-dichloro-1,3-cyclo-pentenedione). The enzyme also has potent catalase activity and in the absence of halide ion, acts as a peroxidase similar to plant peroxidases. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (4); Chain (1); Disulfide bond (1); Frameshift (2); Glycosylation (14); Helix (11); Metal binding (4); Modified residue (1); Mutagenesis (1); Propeptide (1); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Chloride;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Heme;Iron;Manganese;Metal-binding;Oxidoreductase;Peroxidase;Pyrrolidone carboxylic acid;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 21; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:8747463 |
| Post Translational Modification | PTM: N- and O-glycosylated. |
| Signal Peptide | SIGNAL 1..20 |
| Structure 3D | X-ray crystallography (13) |
| Cross Reference PDB | 1CPO; 2CIV; 2CIW; 2CIX; 2CIY; 2CIZ; 2CJ0; 2CJ1; 2CJ2; 2CPO; 2J18; 2J19; 2J5M; |
| Mapped Pubmed ID | 16790441; 17190816; 17211068; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,504 |
| Kinetics | |
| Metal Binding | METAL 50; /note=Iron (heme axial ligand); METAL 125; /note=Manganese; METAL 126; /note=Manganese; via carbonyl oxygen; METAL 129; /note=Manganese |
| Rhea ID | |
| Cross Reference Brenda | 1.11.1.10; |