| IED ID | IndEnz0018001692 |
| Enzyme Type ID | peroxidase001692 |
| Protein Name |
Translocase of chloroplast 34, chloroplastic AtToc34 EC 3.6.5.- 34 kDa chloroplast outer envelope protein GTP-binding protein OEP34 Plastid protein import 3 |
| Gene Name | TOC34 OEP34 PPI3 At5g05000 MUG13.14 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEKAAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVLLYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSNALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWELRDSGLASRRS |
| Enzyme Length | 313 |
| Uniprot Accession Number | Q38906 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 162; /note=GTP; via amide nitrogen; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.6.5.- |
| Enzyme Function | FUNCTION: GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast. {ECO:0000269|PubMed:12741849, ECO:0000269|PubMed:12897258, ECO:0000269|PubMed:15053763, ECO:0000269|PubMed:16435266}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 48..53; /note=GTP; /evidence=ECO:0000250; NP_BIND 67..72; /note=GTP; /evidence=ECO:0000250; NP_BIND 210..211; /note=GTP; /evidence=ECO:0000250 |
| Features | Binding site (1); Chain (1); Domain (1); Initiator methionine (1); Metal binding (2); Modified residue (1); Motif (1); Nucleotide binding (3); Region (7); Sequence conflict (4); Transmembrane (1) |
| Keywords | Acetylation;Chloroplast;GTP-binding;Hydrolase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Plastid;Plastid outer membrane;Protein transport;Receptor;Reference proteome;Transmembrane;Transmembrane helix;Transport |
| Interact With | O81283; Itself; P69249 |
| Induction | INDUCTION: Induced in light but repressed in darkness. {ECO:0000269|PubMed:12897258}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane {ECO:0000269|PubMed:10998188}; Single-pass membrane protein {ECO:0000269|PubMed:10998188}. Note=May contain beta barrel transmembrane regions. |
| Modified Residue | MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22223895 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10373488; 10646606; 12766230; 12938931; 12951325; 14765117; 15028209; 15733839; 15773849; 15842619; 16618929; 18193034; 18400179; 18431481; 18487635; 18493043; 18541539; 19086173; 19188370; 19435889; 19744928; 20444229; 21057194; 21612577; 23118188; 26101739; 28018422; 28559331; 28745032; 29487196; 30521026; 31878089; 9099756; 9405363; 9756470; |
| Motif | MOTIF 287..295; /note=AKR2A-binding sequence (ABS) required for chloroplast outer envelope membrane targeting; /evidence=ECO:0000269|PubMed:21057222 |
| Gene Encoded By | |
| Mass | 34,707 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=130 nM for GDP (at pH 7.4) {ECO:0000269|PubMed:12741849, ECO:0000269|PubMed:17261588}; KM=6.3 uM for GTP (at pH 7.6 and 25 degrees Celsius) {ECO:0000269|PubMed:12741849, ECO:0000269|PubMed:17261588}; Vmax=940 nmol/min/ug enzyme with GTP as substrate (at pH 7.4) {ECO:0000269|PubMed:12741849, ECO:0000269|PubMed:17261588}; |
| Metal Binding | METAL 52; /note=Magnesium; /evidence=ECO:0000250; METAL 70; /note=Magnesium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |