Detail Information for IndEnz0018001695
IED ID IndEnz0018001695
Enzyme Type ID peroxidase001695
Protein Name Glutathione S-transferase YfcF
EC 2.5.1.18
Gene Name yfcF b2301 JW2298
Organism Escherichia coli (strain K12)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12)
Enzyme Sequence MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGEHLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARARQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNLFGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG
Enzyme Length 214
Uniprot Accession Number P77544
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: GSH transferase activity is inhibited by S-hexyl glutathione. {ECO:0000269|PubMed:17018556}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:17018556};
DNA Binding
EC Number 2.5.1.18
Enzyme Function FUNCTION: Exhibits glutathione (GSH) S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB); however this activity is as low as 1% of that of GstA. Also displays a GSH-dependent peroxidase activity toward cumene hydroperoxide. Is involved in defense against oxidative stress, probably via its peroxidase activity. {ECO:0000269|PubMed:17018556}.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Activity decreases as pH is lowered from neutral to acidic. {ECO:0000269|PubMed:17018556};
Pathway
nucleotide Binding
Features Beta strand (4); Chain (1); Domain (2); Helix (9); Mutagenesis (2); Turn (3)
Keywords 3D-structure;Oxidoreductase;Peroxidase;Reference proteome;Transferase
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 3BBY;
Mapped Pubmed ID 16606699;
Motif
Gene Encoded By
Mass 24,326
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.24 mM for glutathione {ECO:0000269|PubMed:17018556}; KM=1.1 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:17018556}; Note=kcat is 0.11 sec(-1) for the GSH transferase reaction with CDNB as substrate.;
Metal Binding
Rhea ID RHEA:16437
Cross Reference Brenda