IED ID | IndEnz0018001695 |
Enzyme Type ID | peroxidase001695 |
Protein Name |
Glutathione S-transferase YfcF EC 2.5.1.18 |
Gene Name | yfcF b2301 JW2298 |
Organism | Escherichia coli (strain K12) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
Enzyme Sequence | MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGEHLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARARQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNLFGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG |
Enzyme Length | 214 |
Uniprot Accession Number | P77544 |
Absorption | |
Active Site | |
Activity Regulation | ACTIVITY REGULATION: GSH transferase activity is inhibited by S-hexyl glutathione. {ECO:0000269|PubMed:17018556}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:17018556}; |
DNA Binding | |
EC Number | 2.5.1.18 |
Enzyme Function | FUNCTION: Exhibits glutathione (GSH) S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB); however this activity is as low as 1% of that of GstA. Also displays a GSH-dependent peroxidase activity toward cumene hydroperoxide. Is involved in defense against oxidative stress, probably via its peroxidase activity. {ECO:0000269|PubMed:17018556}. |
Temperature Dependency | |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Activity decreases as pH is lowered from neutral to acidic. {ECO:0000269|PubMed:17018556}; |
Pathway | |
nucleotide Binding | |
Features | Beta strand (4); Chain (1); Domain (2); Helix (9); Mutagenesis (2); Turn (3) |
Keywords | 3D-structure;Oxidoreductase;Peroxidase;Reference proteome;Transferase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 3BBY; |
Mapped Pubmed ID | 16606699; |
Motif | |
Gene Encoded By | |
Mass | 24,326 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.24 mM for glutathione {ECO:0000269|PubMed:17018556}; KM=1.1 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:17018556}; Note=kcat is 0.11 sec(-1) for the GSH transferase reaction with CDNB as substrate.; |
Metal Binding | |
Rhea ID | RHEA:16437 |
Cross Reference Brenda |