| IED ID | IndEnz0018001706 |
| Enzyme Type ID | peroxidase001706 |
| Protein Name |
NADH peroxidase NPXase Npx EC 1.11.1.1 |
| Gene Name | npr EF_1211 |
| Organism | Enterococcus faecalis (strain ATCC 700802 / V583) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Enterococcaceae Enterococcus Enterococcus faecalis (Streptococcus faecalis) Enterococcus faecalis (strain ATCC 700802 / V583) |
| Enzyme Sequence | MKVIVLGSSHGGYEAVEELLNLHPDAEIQWYEKGDFISFLSCGMQLYLEGKVKDVNSVRYMTGEKMESRGVNVFSNTEITAIQPKEHQVTVKDLVSGEERVENYDKLIISPGAVPFELDIPGKDLDNIYLMRGRQWAIKLKQKTVDPEVNNVVVIGSGYIGIEAAEAFAKAGKKVTVIDILDRPLGVYLDKEFTDVLTEEMEANNITIATGETVERYEGDGRVQKIVTDKNAYDADLVVVAVGVRPNTAWLKGTLELHPNGLIKTDEYMRTSEPDVFAVGDATLIKYNPADTEVNIALATNARKQGRFAVKNLEEPVKPFPGVQGSSGLAVFDYKFASTGINEVMAQKLGKETKAVTVVEDYLMDFNPDKQKAWFKLVYDPETTQILGAQLMSKADLTANINAISLAIQAKMTIEDLAYADFFFQPAFDKPWNIINTAALEAVKQER |
| Enzyme Length | 447 |
| Uniprot Accession Number | P37062 |
| Absorption | |
| Active Site | ACT_SITE 10; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:8756456; ACT_SITE 42; /note=Redox-active; /evidence=ECO:0000269|PubMed:8756456 |
| Activity Regulation | |
| Binding Site | BINDING 32; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; BINDING 42; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; BINDING 132; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; BINDING 160; /note="NAD; via amide nitrogen"; /evidence="ECO:0000269|PubMed:8425532"; BINDING 179; /note="NAD"; /evidence="ECO:0000269|PubMed:8425532"; BINDING 188; /note="NAD"; /evidence="ECO:0000269|PubMed:8425532"; BINDING 243; /note="NAD; via amide nitrogen"; /evidence="ECO:0000269|PubMed:8425532"; BINDING 281; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; BINDING 297; /note="NAD; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:8425532"; BINDING 299; /note="FAD; via amide nitrogen"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; BINDING 328; /note="NAD; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:8425532" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H(+) + H2O2 + NADH = 2 H2O + NAD(+); Xref=Rhea:RHEA:18509, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16240, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.11.1.1; |
| DNA Binding | |
| EC Number | 1.11.1.1 |
| Enzyme Function | FUNCTION: Peroxidase whose active site is a redox-active cysteine-sulfenic acid. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 7..11; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456"; NP_BIND 110..113; /note="FAD"; /evidence="ECO:0000269|PubMed:8425532, ECO:0000269|PubMed:8756456" |
| Features | Active site (2); Beta strand (28); Binding site (11); Chain (1); Helix (15); Modified residue (1); Nucleotide binding (2); Sequence conflict (1); Turn (9) |
| Keywords | 3D-structure;Direct protein sequencing;FAD;Flavoprotein;NAD;Oxidation;Oxidoreductase;Peroxidase;Redox-active center;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 42; /note=Cysteine sulfenic acid (-SOH); /evidence=ECO:0000269|PubMed:8756456 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (8) |
| Cross Reference PDB | 1F8W; 1JOA; 1NHP; 1NHQ; 1NHR; 1NHS; 1NPX; 2NPX; |
| Mapped Pubmed ID | 10956025; 7711038; 7766608; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,566 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18509 |
| Cross Reference Brenda | 1.11.1.1; |