| IED ID | IndEnz0020000014 |
| Enzyme Type ID | mannase000014 |
| Protein Name |
Beta-mannosidase A EC 3.2.1.25 Mannanase A Mannase A |
| Gene Name | mndA NFIA_054490 |
| Organism | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Enzyme Sequence | MHVKAETVLALLTPGLPSVVGQHVVDLSGDGWTLSSTALDRTVPGHLPSQVHLDLFEAGVINITASMILTFVGLLMPIGRIPATRLKACDLENFESTWLVFDGLDTFTTITFCDQHVGSTDNQFRQYHFDVSQILKECKQDPVIRINFGSAPSIANAIAKSPDAEEWPPGVQITNEYPNRWYIRKEQSDFGWDWGPAFAPVGPWKPSYIVQNNHAELYVLNTDLDFYRQGQINYLPPDQSQPWIVNASIDILGPVPWKPSMSIEIKDAATGSVLSSGLLQNVTVSGNSITGTTTIDGDAPKLWWPSGMGEQNLYNVTVTVQNDKKKSLAKVTKRTGFRTIFLNQRNITDDQLAQGIAPGANWHFEINGHAFYAKGSNIIPPDAFWPRVTQARMARLFDAVTAGNQNMLRVWASGAYLHDFIYDLADEKGILLWSEFQFSDALYPVNDAFLENVAAEVVYNVRRVNHHPSLALWAGGNEIESLMLPMVKRADPTGYSKYVGEYEKLYISLILPLVYENTRSITYSPSSTTEGYLYVNLSAPVPMAERYSNTTPGSYYGDTDYYNYDTSVSFDYNHYPVGRFANEFGFHSMPSLQTWQQAVDPEDLHFNSSVVMLRNHHYTAGGLFTDNFKNSSKGMGEMTMGVEAYYPIPSKSDSVANFSAWCHATQLFQADMYKSQIQFYRRGSGMPERQLGSLYWQLEDIWQAPTWAGIEYDGRWKVLHYVARDIYQPIIVSPFWNYTTGRLEVYVTSDLWEPAQGTVNLTWVDLSGKTIANNAGTPETVNFTVGALNTTNIYTTNISELSLPDLKDSILILSLSGEGRLPNTSSKKAFVHQNHFTPVFPKDLSLKDPKLEISYSPESRKFTVQATGGVSLYTWLDYPAGAVGYFEENAFVLLPGVQKEVSFAAQEGNVTDDWVRRVTVQSLWDQKVRD |
| Enzyme Length | 930 |
| Uniprot Accession Number | A1DMT2 |
| Absorption | |
| Active Site | ACT_SITE 478; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.; EC=3.2.1.25; |
| DNA Binding | |
| EC Number | 3.2.1.25 |
| Enzyme Function | FUNCTION: Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Involved in the degradation of polymeric mannan and galactomannan (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; N-glycan degradation. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (16); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 104,276 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |