| IED ID | IndEnz0020000023 |
| Enzyme Type ID | mannase000023 |
| Protein Name |
Beta-mannosidase B EC 3.2.1.25 Mannanase B Mannase B |
| Gene Name | mndB ACLA_066240 |
| Organism | Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus clavatus Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
| Enzyme Sequence | MAALQRFPLSKGWSFKDSEDKSEDAWMPVPVVPSVVQQDLQANNKLKDPYIGFNELETRWVNEKSWTYKTTFQKPAVPAGSAIFLAFDGLDTFATVKLDGNVILESDNMFLAHRLDVTKALEAEGDHSLEIDFDCAFLRAKELRKQDSKHNWASFNGDPSRLSVRKSQYHWGWDWGPVLMTAGIWREVRLEVYTARVADLWTDVQLASDHQNAQITAFAEVESVNSDAHKARFTLSLHGQELGREEVSVSEDGSAKVSFDVKSPSLWWPHGYGDATLYEVSVSLVKDQDEVHQVSKKFGIRTAEVVQQPDKHGKSFFFRVNGVDIFCGGSCWIPADNYLPSVTADRYRKWIELMVHGRQVMIRVWGGGNYEDDSFYDACDELGVLVWQDFMFGCGNYPTWPNLLESIRKESVYNVRRLRHHPSIVVWVGNNEDYQVQESAGLTYDFEDKNPENWLKTDFPARYIYEKILPEVVEEYSPSTFYHPGSPWGDGKTTSDPTVGDMHQWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIDYFVENEADKFPQSHVLDFHNKADGHERRIATYLVENLRTATDLETHVYLTQVVQAETMMFGYRGWRRQWGDERHCGGALLWQLNDCWPTISWAIVDYFLRPKPAFYAVARVLNPIAVGVRREHHDWSVTHAQPPKTSKFELWVASSLQKEVQGTVELRFLSINTGLEVRERIVHENVSIVPNGTTNLIVDGLIDYKVHPEPHVLAVRIWVNGELVARDVDWPQPFKYLDLSNRGLEVKLVSESENEQTLLLSAQKPVKCLVFEEREGVRISDSAIDIVPGDEQRVTIKGMKPGDAPLKYKFLGQ |
| Enzyme Length | 845 |
| Uniprot Accession Number | A1CGA8 |
| Absorption | |
| Active Site | ACT_SITE 432; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.; EC=3.2.1.25; |
| DNA Binding | |
| EC Number | 3.2.1.25 |
| Enzyme Function | FUNCTION: Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers mannobiose over mannotriose and has no activity against polymeric mannan. Is also severely restricted by galactosyl substitutions at the +1 subsite (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; N-glycan degradation. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (2) |
| Keywords | Carbohydrate metabolism;Glycoprotein;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 96,760 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |