| IED ID | IndEnz0020000026 |
| Enzyme Type ID | mannase000026 |
| Protein Name |
Beta-mannosidase B EC 3.2.1.25 Mannanase B Mannase B |
| Gene Name | mndB AO090010000208 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MAAFSQYPLSTGWSFKDSDDQSPEAWMPVPVVPSVAHQDLQANQKLKNPYIGFNELDARWVNDKSWTYRTVFQKPAVAAGSSIILAFDGLDTFATVKLDGSVILQSDNMFLAHRVDVTKALEAEGDHVLEIDFDCAMRRARELREKDTKHNWASFNGDPARMAVRKAQYHWGWDWGPLLSTAGIWREVRLEVYSAKISDLWTEVELASDHQTARVSAFAEVDAADSVDSYKASFLLSLHGKEVAREVATLKDKVANVTFDVTQPSLWWPNGYGDPALYEISVSLEKEDCEIHSVSKKIGIRTAELIQQPDRHGKSFFFRINGVDVFCGGSCWIPADNLLPSITAERYRKWIELMVAGRQVMIRVWGGGCYEDDSFYQACDELGVLVWQDFMFGCGNYPTWPELLESIEKEANYNVRRLRHHPSIVVYVGNNEDYQVQESAGLVYDYEDKNPENWLKTDFPARYIYEKLLPSVVEKLSPKTVYHPGSPWGDGKITSDPTVGDMHQWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIEYFVENEADKYPQSHVLDFHNKADGHERRIATYLVENLRTATDLETYVYLTQVVQAETMMFGYRGWRRQWGDERHCGGALLWQLNDCWPTISWAIVDYFLRPKPAFYAVARVLKPIAVGVRREHHDWSVTHAQPPKTSKYELWIASSLQKETVGTIELRFLSVNTGLDVRAPILRDNVKIVPNGTTNILEGVIDHKAQPEPHVLAARLWVDGEVTARDVDWPQPFKYLDLSDRGLEVNKVSESGNEQKLLITAKKPVKCLVFEERDGIRVSDSAMDIVPGDGQTVTVTGLKAGDAPLKYKYLGQ |
| Enzyme Length | 844 |
| Uniprot Accession Number | Q2TXB7 |
| Absorption | |
| Active Site | ACT_SITE 432; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.; EC=3.2.1.25; |
| DNA Binding | |
| EC Number | 3.2.1.25 |
| Enzyme Function | FUNCTION: Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers mannobiose over mannotriose and has no activity against polymeric mannan. Is also severely restricted by galactosyl substitutions at the +1 subsite (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan metabolism; N-glycan degradation. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 95,960 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |