The P3 /AgNPs sensor was chosen for the detection of Aβ1-42 and human Insulin (HI) . The limit of detection (LoD) was found to be 76×10-18 M and 26×10-18 M for Aβ1-42 and HI, respectively. The sensitivity achieved is one order improved for Aβ1-42 and four orders for HI when compared with reported values