| Effects |
(1) We took advantage of the TRITC–CHP/CHPNH2 and measured the Förster resonance energy transfer (FRET) between both the TRITC-labelled nanogel derivative and FITC-labelled Aβ in vitro,CHPNH2 was more effective than CHP at quenching the fluorescence of the donor (Monomers: CHP = 0.23 ± 0.01 vs. CHPNH2 = 0.48 ± 0.02; Oligomers: CHP = 0.34 ± 0.01 vs. CHPNH2 = 0.57 ± 0.01 p < 0.01) at the lowest concentration. This result suggests that the distance between FITC and TRITC was less than 100 Å, indicating a direct interaction between CHPNH2 and Aβ at this concentration.(2) Neural cells exposed to unmodified CHP (12 nm) induced a small, but significant proliferative effect, whereas CHPNH2 (12 nm) led to a cell loss (33 ± 7%; *p < 0.05) .(3) N9 cells were pre-treated with CHP/CHPNH2, following which Aβ(1–42) -oligomers (Aβolig) were then administered for 24 hours (Fig. 6a) . CHP completely rescued microglia from Aβolig-mediated cell death, while CHPNH2 attained a partial rescue (84 ± 1%; significant at p < 0.01 relative to control and Aβolig) |
