| Effects |
(1) We first evaluated the safety of α-M/NP-α-M treatment at this dose in vivo(2) the mice were sacrificed and the brain slices were immunostained with anti-AT8 or anti-Aβ antibodies. Compared with other mice, NP-α-M treated mice had significantly reduced PHF and Aβ depositions in the cortex and hippocampus (3) in the NP-α-M treated mice, most neurons remained light staining and round shape, which suggested that NP-α-M ameliorated the neuron damage in the 3 × TG mice(4) measured some typical pro-inflammatory factors by ELISA, and found that NP-α-M reduced TNF-α, IL-1β, and IL-6 levels both in the hippocampus and cortex(5) In the open field test (OFT) , the moving distance and time of the NP-α-M treated mice and the saline treated mice had no difference , indicating that NP-α-M did not induce psychostimulant disturbances in the mice. |
