(1) The cell viability as well as the integrity of the endothelial cells was not impaired by the nanoparticulate flurbiprofen;(2) The nanoparticulate flurbiprofen reduced the amount of the Aβ42 peptides in a concentration dependent manner . The levels of Aβ40 remained unaffected. The decrease of the Aβ42 levels by the PLA-flurbiprofen nanoparticles is achieved by the in vitro release of the drug from the nanoparticles;(3) When flurbiprofen-loaded nanoparticles were added luminally to the endothelial cells, Aβ42 levels decreased in a concentration dependent manner whereas the levels of Aβ40 remained unchanged, the nanoparticles were transported in this in vitro BBB model and that the embedded flurbiprofen was able to reduce Aβ42 levels by modulating the γ-secretase activity.
Research Models
Vitro BBB model;The mouse brain endothelial cell line bEnd.3
